MicroRNA-155-3p promotes hepatocellular carcinoma formation by suppressing FBXW7 expression.
MicroRNA-155-3p promotes hepatocellular carcinoma formation by suppressing FBXW7 expression.
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MicroRNA-155-3p通过抑制FBXW7表达促进肝细胞癌形成
DOI：
10.1186/s13046-016-0371-6
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发表时间：
2016-06-16
期刊：
影响因子：
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通讯作者：
He S
中科院分区：
文献类型：
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作者：
Tang B;Lei B;Qi G;Liang X;Tang F;Yuan S;Wang Z;Yu S;He S
背景MicroRNA (miRNA) 是一种小非编码 RNA，在人类恶性肿瘤中经常失调。在本研究中，我们分析了 miR-155-3p 在肝细胞癌 (HCC) 中的作用，据报道 HCC 参与了一些其他类型的癌症。方法采用 qRT-PCR 测量 HCC 标本和 HCC 细胞系中 miR-155-3p 的水平。将 miR-155-3p 和 miR-155-3p 抑制剂的过表达转染至 HCC 细胞系中，以研究其在 HCC 中的作用。使用集落形成测定和3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑(MTT)测定来分析体外细胞增殖。在 BALB/c 裸鼠中进行体内肿瘤形成测定。进行荧光素酶报告基因检测来测量 F-Box 和 WD 重复序列包含 7 (FBXW7) 的翻译。结果我们发现 miR-155-3p 在 HCC 组织和细胞系中均显着上调。 miR-155-3p 的过表达可增强体外 HCC 细胞增殖和体内肿瘤发生。此外，miR-155-3p的过表达主要通过抑制FBXW7的表达与FBXW7水平降低相关。结论我们的研究表明miR-155-3p在HCC的发病机制中发挥重要作用，并暗示其在HCC癌症治疗中的潜在应用。
BackgroundMicroRNAs (miRNAs) are small non-coding RNAs frequently dysregulated in human malignant tumors. In the present study, we analyzed the role miR-155-3p plays in Hepatocellular carcinoma (HCC), which has been reported participation in some other types of cancer.MethodsqRT-PCR was used to measure the levels of miR-155-3p in HCC specimens and HCC cell lines. Overexpression of miR-155-3p and miR-155-3p inhibitor were transfected into HCC cell lines to investigate its role in HCC. Colony formation assay and 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays were used to analyses cell proliferation in vitro. In vivo tumor formation assays were performed in BALB/c nude mice. Luciferase reporter assay was carried out to measure the translation of F-Box and WD repeat romain containing 7 (FBXW7).ResultsWe found that miR-155-3p was remarkably upregulated both in HCC tissue and cell lines. Overexpression of miR-155-3p enhanced HCC cell proliferation in vitro and tumorigenesis in vivo. In addition, overexpression of miR-155-3p is correlated with decreased levels FBXW7 mainly through inhibiting the expression of FBXW7.ConclusionsOur studies suggest that miR-155-3p plays an important role in the pathogenesis of HCC and implicates its potential applications in the treatment of HCC cancer.