Transcriptomic and functional analysis of the Anopheles gambiae salivary gland in relation to blood feeding
Transcriptomic and functional analysis of the Anopheles gambiae salivary gland in relation to blood feeding
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DOI：
10.1186/1471-2164-11-566
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发表时间：
2010-10-14
期刊：
影响因子：
4.4
通讯作者：
Dimopoulos, George
中科院分区：
文献类型：
--
作者：
Das, Suchismita;Radtke, Andrea;Dimopoulos, George
背景：冈比亚按蚊唾液腺在疟疾传播中发挥着重要作用，并表达多种生物活性成分，通过防止脊椎动物宿主探测部位的血小板聚集、凝血、血管舒张以及炎症和其他反应来促进血液喂养。结果：我们对冈比亚按蚊唾液腺对吸血的反应进行了全局转录组分析，以确定参与吸血的候选基因。总共有 4,978 个基因被发现在该组织中转录。对喂血前后唾液腺转录组的比较发现，分别有 52 个和 41 个转录本显着上调和下调。进一步选择了 10 个基因，使用 RNAi 介导的基因沉默方法来评估它们在血液喂养过程中的作用。编码 D7L2、按蚊素、过氧化物酶、SG2 前体和 5' 核苷酸酶基因的唾液腺基因的耗竭显着增加了冈比亚蚊子的探测时间，从而提高了它们吸血的能力。结论：唾液腺转录组约占蚊子总转录组的 38%，其中一小部分在两小时后就已经因吸血而发生动态变化。更好地了解唾液腺转录组及其功能有助于制定病原体传播控制策略和鉴定医学相关的生物活性化合物。
Background: The Anopheles gambiae salivary glands play a major role in malaria transmission and express a variety of bioactive components that facilitate blood-feeding by preventing platelet aggregation, blood clotting, vasodilatation, and inflammatory and other reactions at the probing site on the vertebrate host.Results: We have performed a global transcriptome analysis of the A. gambiae salivary gland response to blood-feeding, to identify candidate genes that are involved in hematophagy. A total of 4,978 genes were found to be transcribed in this tissue. A comparison of salivary gland transcriptomes prior to and after blood-feeding identified 52 and 41 transcripts that were significantly up-regulated and down-regulated, respectively. Ten genes were further selected to assess their role in the blood-feeding process using RNAi-mediated gene silencing methodology. Depletion of the salivary gland genes encoding D7L2, anophelin, peroxidase, the SG2 precursor, and a 5'nucleotidase gene significantly increased probing time of A. gambiae mosquitoes and thereby their capacity to blood-feed.Conclusions: The salivary gland transcriptome comprises approximately 38% of the total mosquito transcriptome and a small proportion of it is dynamically changing already at two hours in response to blood feeding. A better understanding of the salivary gland transcriptome and its function can contribute to the development of pathogen transmission control strategies and the identification of medically relevant bioactive compounds.