Cathepsin O is involved in the innate immune response and metamorphosis of Antheraea pernyi
Cathepsin O is involved in the innate immune response and metamorphosis of Antheraea pernyi
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组织蛋白酶 O 参与柞蚕的先天免疫反应和变态
DOI：
10.1016/j.jip.2017.08.015
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发表时间：
2017
影响因子：
3.4
通讯作者：
Guo-Qing Wei
中科院分区：
文献类型：
--
作者：
Yu-Xuan Sun;Chao-liang Liu;Guo-Qing Wei
组织蛋白酶是哺乳动物木瓜蛋白酶样半胱氨酸蛋白酶的关键成员，在免疫应答中发挥重要作用。本研究从柞蚕组织蛋白酶O蛋白酶（ApCathepsin O）基因组中克隆了一个cDNA片段。该基因的开放阅读框为1170 bp，编码390个氨基酸残基的蛋白质，包括一个保守的I29抑制剂结构域和一个肽酶C1 A结构域。与其他以前报道的组织蛋白酶O蛋白的比较显示，从45%到79%的范围内的同一性。实时荧光定量PCR（qRT-PCR）和Western blot分析表明，ApCathepsin O在脂肪体中高表达，蛹期高表达表明其可能参与变态过程。在暴露于四种不同的热灭活病原体（大肠杆菌、白僵菌、藤黄微球菌和A. pernyi核型多角体病毒）感染后，ApCathepsin O mRNA的表达水平显著升高，并表现出不同的表达模式。这表明ApCathepsin O可能参与了A. pernyi。有趣的是，注射20-羟基蜕皮激素（20 E）后，ApCathepsin O表达上调，这表明它可能受到20 E的调节。结论：ApCathepsin O是一种蛋白酶，可能在A. pernyi。
Cathepsins are key members of mammalian papain-like cysteine proteases that play an important role in the immune response. In this study, a fragment of cDNA encoding cathepsin O proteinase (ApCathepsin O) was cloned from Antheraea pernyi . It contains an open reading frame of 1170 bp and encodes a protein with 390 amino acid residues, including a conserved I29 inhibitor domain and a peptidase C1A (clan CA of cysteine proteases, papain family C1 subfamily) domain. Comparison with other previously reported cathepsin O proteins showed identity ranging from 45% to 79%. Quantitative real-time PCR (qRT-PCR) and Western blot analysis revealed that ApCathepsin O was highly expressed in the fat body; furthermore, the high expression during the pupal stage indicated that it might be involved during metamorphosis. After exposure to four different heat-killed pathogens ( Escherichia coli , Beauveria bassiana , Micrococcus luteus , and A. pernyi nucleopolyhedrovirus), the expression levels of ApCathepsin O mRNA significantly increased and showed variable expression patterns. This indicates that ApCathepsin O is potentially involved in the innate immune system of A. pernyi. Interestingly, ApCathepsin O expression was upregulated after 20-hydroxyecdysone (20E) injection, which suggested that it might be regulated by 20E. In conclusion, ApCathepsin O is a protease that may play an important role in the innate immune response and metamorphosis of A. pernyi.