Identification of rate-limiting steps in yeast heme biosynthesis
Identification of rate-limiting steps in yeast heme biosynthesis
复制标题
DOI：
10.1016/j.bbrc.2003.09.151
复制
发表时间：
2003-10-31
影响因子：
3.1
通讯作者：
Rytka, J
中科院分区：
文献类型：
--
作者：
Hoffman, M;Góra, M;Rytka, J
血红素在酿酒酵母中的生物合成途径是一个高度调控的系统，但其调控机制尚不清楚。在试图确定血红素合成的限速步骤，这可能构成潜在的监管点，我们构建了酵母菌株过量生产的两种酶的途径：胆色素原合成酶（PBG-S）和脱氨酶（PBG-D）。生化分析的酶过度生产菌株显示细胞内的胆色素原和卟啉积累。这些结果表明，这两种酶在酵母血红素生物合成中起限速作用。(C)2003年爱思唯尔公司All right reserved.
The heme biosynthesis pathway in the yeast Saccharomyces cerevisiae is a highly regulated system, but the mechanisms accounting for this regulation remain unknown. In an attempt to identify rate-limiting steps in heme synthesis, which may constitute potential regulatory points, we constructed yeast strains overproducing two enzymes of the pathway: the porphobilinogen synthase (PBG-S) and deaminase (PBG-D). Biochemical analysis of the enzyme-overproducing strains revealed intracellular porphobilinogen and porphyrin accumulation. These results indicate that both enzymes play a rate-limiting role in yeast heme biosynthesis. (C) 2003 Elsevier Inc. All right reserved.