Atherosclerotic neovasculature MR imaging with mixed manganese-gadolinium nanocolloids in hyperlipidemic rabbits.
Atherosclerotic neovasculature MR imaging with mixed manganese-gadolinium nanocolloids in hyperlipidemic rabbits.
复制标题
使用混合锰钆纳米胶体对高脂血症兔进行动脉粥样硬化新生血管 MR 成像。
DOI：
10.1016/j.nano.2014.12.008
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Lanza GM
中科院分区：
文献类型：
--
作者：
Wang K;Pan D;Schmieder AH;Senpan A;Caruthers SD;Cui G;Allen JS;Zhang H;Shen B;Lanza GM
开发了一种高r1弛豫率的锰钆纳米胶体（αvβ3-MnOL-Gd NC），并使用临床MRI扫描仪（3 T）有效地检测了喂养高胆固醇饮食12个月的家兔的动脉粥样硬化血管生成。新生血管信号强度的3D标测显示了斑块血管生成扩张的空间一致性和强度，这可能与其他高风险MR生物指标一起有助于识别中度（40%至60%）血管狭窄的高风险患者。显微镜检查证实了荧光αvβ3-MnOL-Gd NC的主要介质和斑块分布，反映了MR数据。观察到αvβ3-整联蛋白新生血管和巨噬细胞的预期密切空间相关性，特别是在斑块肩部区域。油酸锰通过胆汁系统进入粪便进行生物消除。Gd-DOTA通过胆汁-粪便和肾脏排泄途径消除。αvβ3-MnOL-Gd NC为动脉粥样硬化的T1 w新生血管成像提供了有效的载体。基于αvβ3-MnOLGd NC分子成像的高血压兔（12个月）降胸主动脉内血管生成指数的三维（3D）MR重建（3 T），显示内膜下明显的新生血管。例如，这种方法结合目前的斑块不稳定性的MR生物标志物可以用于中度颈动脉疾病患者的风险分层治疗护理。
A high r1 relaxivity manganese-gadolinium nanocolloid (αvβ3-MnOL-Gd NC) was developed and effectively detected atherosclerotic angiogenesis in rabbits fed cholesterol-rich diets for 12 months using a clinical MRI scanner (3T). 3D mapping of neovasculature signal intensity revealed the spatial coherence and intensity of plaque angiogenic expansion, which may, with other high risk MR bioindicators, help identify high-risk patients with moderate (40% to 60%) vascular stenosis. Microscopy confirmed the predominant media and plaque distribution of fluorescent αvβ3-MnOL-Gd NC, mirroring the MR data. An expected close spatial association of αvβ3-integrin neovasculature and macrophages was noted, particularly within plaque shoulder regions. Manganese oleate bioelimination occurred via the biliary system into feces. Gd-DOTA was eliminated through the bile-fecal and renal excretion routes. αvβ3-MnOL-Gd NC offers an effective vehicle for T1w neovascular imaging in atherosclerosis. Three-dimensional (3D) MR reconstruction (3T) of angiogenesis index within the descending thoracic aorta in hyperlipidemic rabbit (12 month) based on molecular imaging with αvβ3-MnOLGd NC illustrating marked neovascularity subjacent to intima. For example, such an approach in conjunction with current MR biomarkers of plaque instability could be used to risk-stratify therapeutic care in patients with moderate carotid disease.