Innervation of stem cell-derived neurons into auditory epithelia of mice
Innervation of stem cell-derived neurons into auditory epithelia of mice
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DOI：
10.1097/00001756-200505310-00001
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发表时间：
2005-05
期刊：
影响因子：
1.7
通讯作者：
Masahiro Matsumoto;T. Nakagawa;Toru Higashi;Tae-soo Kim;K. Kojima;Tomoko Kita;T. Sakamoto;J. Ito
中科院分区：
文献类型：
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作者：
Masahiro Matsumoto;T. Nakagawa;Toru Higashi;Tae-soo Kim;K. Kojima;Tomoko Kita;T. Sakamoto;J. Ito
这项研究探讨了胚胎干细胞来源的神经元作为细胞移植治疗原发螺旋神经节神经元丢失的潜力。为了评价胚胎干细胞来源的神经元向听觉上皮细胞的神经支配能力，将其与小鼠的听觉上皮组织块共培养7d。组织学分析显示，神经突起从胚胎干细胞来源的神经元向听觉毛细胞大量延长。胚胎干细胞来源的神经突起与毛细胞相邻或围绕毛细胞，并表达突触素，突触小泡的标志。这些发现证明了胚胎干细胞来源的神经元具有向听觉上皮细胞再支配的能力，表明胚胎干细胞来源的神经元作为替代螺旋神经节神经元的移植具有很高的潜力。
This study examined the potential of embryonic stem cell-derived neurons as transplants for cell therapy for the primary loss of spiral ganglion neurons. To assess the ability of embryonic stem cell-derived neurons for innervation into auditory epithelia, they were cocultured with auditory epithelium explants of mice for 7 days. Histological analysis demonstrated massive elongation of neurites from embryonic stem cell-derived neurons toward auditory hair cells. Embryonic stem cell-derived neurites were adjacent to or surrounding hair cells, and exhibited expression of synaptophysin, a marker for synaptic vesicles. These findings demonstrate the ability of embryonic stem cell-derived neurons for reinnervation into auditory epithelia, indicating a high potential of embryonic stem cell-derived neurons as transplants for replacing spiral ganglion neurons.