Electrochemical Investigation of Isoprenoid Quinone Productions by Shewanella oneidensis MR-1 Detected by Its Destructive Adsorption on an Indium-Tin-Oxide Electrode,
Electrochemical Investigation of Isoprenoid Quinone Productions by Shewanella oneidensis MR-1 Detected by Its Destructive Adsorption on an Indium-Tin-Oxide Electrode,
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通过氧化铟锡电极上的破坏性吸附检测希瓦氏菌 MR-1 产生类异戊二烯醌的电化学研究，
DOI：
10.1149/2.1251610jes
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
T. Nagaoka*
中科院分区：
文献类型：
--
作者：
A. Morishita;S. Higashimae;A. Nomoto;H. Shiigi;T. Nagaoka*
泛醌和甲基萘醌在微生物呼吸链中起重要作用。S. oneidensis是兼性厌氧菌，已知这种微生物需要甲基萘醌（MK）和/或甲基萘醌（MMK）在厌氧条件下还原无机和有机化合物，如Fe (III), Mn (IV)，富马酸盐和硝酸盐。在这篇文章中，我们证明伏安法可以通过加热沉积在氧化铟锡电极上的微生物（热裂解）来定量位于细胞质膜上的泛醌（UQ）， MK和MMK。结果发现，该菌在好氧条件下主要生产UQ，而在厌氧条件下则转向生产MMK。这种转变发生在溶解氧浓度为~ 0.3 mg L−1时。本研究还发现，在营养培养液中，单个细胞的总醌浓度（UQ+ MK+ MMK）在培养的各个阶段都是恒定的，这表明细胞质膜被醌饱和。研究了枸杞对柠檬酸铁（III）的还原作用。结果发现，在完成还原后，该微生物将UQ和MMK水平分别降低至初始值的45%和39%。
Ubiquinone and menaquinone play important roles in microbial respiratory chains. S. oneidensis is a facultative anaerobe, and it has been known that this microbe requires menaquinone (MK) and/or methylmenaquinone (MMK) to reduce inorganic and organic compounds, such as Fe (III), Mn (IV), fumarate, and nitrate, under anaerobic conditions. In this article, we show that voltammetry is useful to quantify ubiquinone (UQ), MK and MMK located in the cytoplasmic membrane simply by heating the microbe (thermal lysis) deposited on an indium-tin oxide electrode. It was found that the microbe predominantly produced UQ under aerobic conditions, while the production was switched to that of MMK under anaerobic conditions. This transition occurred at a dissolved oxygen concentration of∼ 0.3 mg L− 1. It was also found in this study that the total quinone concentration (UQ+ MK+ MMK) in a single cell was constant at all stages of the culture in a nutrition broth, suggesting that the cytoplasmic membrane was saturated with the quinones. The reduction of Fe (III) citrate with S. oneidensis was also studied. It was found that this microbe decreased the UQ and MMK levels to 45% and 39% of the initial values, respectively, after finishing the reduction.