Immunohistological localization of three basement membrane components in various forms of epidermolysis bullosa
Immunohistological localization of three basement membrane components in various forms of epidermolysis bullosa
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不同形式的大疱性表皮松解症中三种基底膜成分的免疫组织学定位
DOI：
10.1111/j.1600-0560.1982.tb01068.x
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发表时间：
1982
影响因子：
1.7
通讯作者：
E. Savolainen
中科院分区：
文献类型：
--
作者：
M. Kero;L. Peltonen;J. Foidart;E. Savolainen
应用免疫组织化学方法，对8例大疱性表皮松解症(EB)患者的皮肤活检标本进行了免疫组织化学研究。所有这些分子要么是基底膜成分，要么是紧密结合的物质。在两种类型的EB单纯型(表皮松解型的亚型)中，剪接发生在基底膜之上，而所有三种抗体的染色都定位于水泡的底部。单纯EB疱疹病毒的疱疹变种被证明是连接的，即分离发生在透明板内。1例临床分类为交界性EB组的患者，被发现患有表皮松解型疾病，在该病例中，所有抗体仅定位于水泡的底部。在皮肤松解型EB患者中，所有抗体都染色了水泡的顶部。免疫荧光技术快速、简便，可作为常规临床诊断的有用方法。
The skin biopsies of eight epidermolysis bullosa (EB) patients, representing epidermolytic, junctional and dermolytic forms of the disease were studied immunohistochemically using antibodies against collagen Types IV and V and a proteoglycan. All these molecules arc either basement membrane components or closely associated substances. In two types of EB simplex (subtype of the epidermolytic form) the splicing took place above the basement membrane, whereas the staining with all three antibodies remained localized to the floor of the blister The herpetiform variant of EB simplex proved to be junctional, i.e. the separation occurred within the lamina lucida. One patient clinically classified as belonging to the junctional EB group, was found to have the epidermolytic form of the disease In this case all antibodies were localized only on the floor of the blister. In the patients with the dermolytic form of EB, all the antibodies stained the roof of the blister. The immunofluorescence techniques are rapid and easy to perform and are therefore proposed as useful for routine clinical diagnosis.