Failure to Replicate an Association of a LINE-1 Element in ERI1 Exoribonuclease Family Member 3 (ERI3) with Schizophrenia.
Failure to Replicate an Association of a LINE-1 Element in ERI1 Exoribonuclease Family Member 3 (ERI3) with Schizophrenia.
复制标题
未能复制 ERI1 核糖核酸外切酶家族成员 3 (ERI3) 中的 LINE-1 元件与精神分裂症的关联。
DOI：
10.1038/npp.2017.145
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Berrettini,WadeH
中科院分区：
文献类型：
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作者：
Doyle,GlennA;Berrettini,WadeH
我们试图在PDS和对照组的第二组血液DNA样本中复制我们最初的发现。从罗格斯大学细胞和DNA库（Infinite Biologics，皮斯卡特维，NJ，USA）获得来自满足精神分裂症的DSM-IV标准的欧美受试者（n= 1665）和对照（n= 997）的EBV转化的类淋巴母细胞系的去识别DNA。在基因分型实验中使用了在无菌水中的20 ng/μl的2微升DNA。如所述（Doyle et al，2017），使用基于Taqman的等位基因区分试验对这些额外的PDS和对照样品进行基因分型，以确定其中是否存在LINE-1元件。
We sought to replicate our original finding in a second set of blood DNA samples from PDS and controls. De-identified DNA from EBV-transformed lymphoblastoid cell lines of European-American subjects who met DSM-IV criteria for schizophrenia (n= 1665) and controls (n= 997) were acquired from the Rutgers University Cell and DNA Repository (Infinite Biologics, Piscataway, NJ, USA). Two microliters of DNA at 20ng/μl in sterile water were used in genotyping experiments. A Taqman-based allelic discrimination assay was used, as described (Doyle et al, 2017), to genotype these additional PDS and control samples for the presence or absence of the LINE-1 element within