In vitro stimulation of Na+-K+-ATPase activity and ouabain binding by cortisol in coho salmon gill.
In vitro stimulation of Na+-K+-ATPase activity and ouabain binding by cortisol in coho salmon gill.
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银鲑鱼鳃中皮质醇对 Na-K-ATP 酶活性和哇巴因结合的体外刺激。
DOI：
10.1152/ajpregu.1989.256.3.r707
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
H. Bern
中科院分区：
文献类型：
--
作者：
S. McCormick;H. Bern
为研究鲑鱼鳃Na+-K+-三磷酸腺苷酶(ATPase)(钠泵)的激素调控作用，建立了一种长达4d的鳃原丝培养技术。台盼蓝拒染率>99.9%，细胞组织学形态正常，培养2~4d的鳃细丝总[Na+]、[K+]和蛋白质含量与分离后即刻水平无明显变化。在鳃Na+-K+-ATPase活性较低的鱼类(蜕皮前)中，皮质醇(0.1、1.0和10.0微克/毫升)在培养4天后引起鳃Na+-K+-ATPase活性显著高于初始水平(41%)和对照水平(45%)，且呈剂量依赖关系。在最初具有较高鳃Na+-K+-ATPase活性(后)的鱼类中，皮质醇部分阻止了在培养4天期间出现的活性下降。类固醇提高鳃Na+-K+-ATPase活性的相对能力是地塞米松大于皮质醇=11-脱氧皮质醇大于皮质松。胰岛素(0.1、1.0和10.0微克/毫升)单独或联合皮质醇对鳃Na+-K+-ATPase活性无显著影响。皮质醇处理显著提高了鳃组织中[~3H]哇巴因的最大结合量(从2.92 pmol/mg干重增加到5.22 pmol/mg干重)，但对解离常数没有显著影响。这些结果表明，皮质醇对硬骨鳃的渗透调节生理有直接影响。
To investigate the hormonal control of gill Na+-K+-adenosinetriphosphatase (ATPase) (the sodium pump) in coho salmon, a technique for the culture of primary gill filaments for up to 4 days was developed. Trypan blue exclusion was greater than 99.9%, histological appearance of the cells was normal, and total [Na+], [K+], and protein content of gill filaments cultured for 2-4 days was unchanged from initial levels (measured immediately after isolation). In fish with initially low gill Na+-K+-ATPase activity (presmolts), cortisol (0.1, 1.0, and 10.0 micrograms/ml) caused a significant dose-dependent increase in gill Na+-K+-ATPase activity over initial (41%) and control levels (45%) after 4 days in culture. In fish with initially high gill Na+-K+-ATPase activity (postsmolts), cortisol partially prevented the decline in activity that occurred during 4 days of culture. The relative ability of steroids to increase gill Na+-K+-ATPase activity was dexamethasone greater than cortisol = 11-deoxycortisol greater than cortisone. Insulin (0.1, 1.0, and 10.0 micrograms/ml), alone or in combination with cortisol, had no significant effect on gill Na+-K+-ATPase activity. Cortisol treatment significantly increased maximum binding capacity of [3H]ouabain in gill tissue (from 2.92 to 5.22 pmol/mg dry wt) but had no significant effect on the dissociation constant. These results demonstrate that cortisol has direct effects on the osmoregulatory physiology of the teleost gill.