SEQUENCE-DEPENDENT HELICAL PERIODICITY OF DNA
SEQUENCE-DEPENDENT HELICAL PERIODICITY OF DNA
复制标题
DOI：
10.1038/292378a0
复制
发表时间：
1981-01-01
期刊：
影响因子：
64.8
通讯作者：
KLUG, A
中科院分区：
文献类型：
--
作者：
RHODES, D;KLUG, A
本文介绍了一种测量溶液中随机序列DNA螺旋周期性的新方法。它包括将短而硬的DNA片段结合到各种平面上，并使用DNA酶I探测最容易到达的磷酸二酯键。以高精度测量产生的片段的长度差异，以直接给出螺旋重复序列。这种方法被扩展到使用另一种酶，微球菌核酸酶和DNase I来研究确定序列DNA的概率。聚（dA-dT）的螺旋重复显然是10.5 ±。0.1<碱基对（bp），该值非常接近于先前对随机序列DNA发现的值，10.6 ±。0.1 BP.聚（dA）·聚（dT）显示出明显不同的螺旋重复，10.0 ± 1.0。0.1 BP.
A new approach to measuring the helical periodicity of random sequence DNA in solution is described. It consists of binding short, stiff pieces of DNA to various flat surfaces and using DNase I to probe the most accessible phosphodiester bonds. The differences in length of the fragments produced are measured with high precision to give the helical repeat directly. This approach was extended to study the preiodicity of defined-sequence DNA using another enzyme, micrococcal nuclease and DNase I. The helical repeat of poly(dA-dT) is apparently 10.5 .+-. 0.1 < base pairs (bp), a value very close to that found earlier for random-sequence DNA, 10.6 .+-. 0.1 bp. Poly(dA).cntdot.poly(dT) exhibits a distinctly different helical repeat, 10.0 .+-. 0.1 bp.