GENERATION OF FUNCTIONAL MURINE MACROPHAGE LINES EMPLOYING A HELPER-FREE AND REPLICATION-DEFECTIVE SV40-RETROVIRUS - CYTOKINE-DEPENDENT GROWTH
GENERATION OF FUNCTIONAL MURINE MACROPHAGE LINES EMPLOYING A HELPER-FREE AND REPLICATION-DEFECTIVE SV40-RETROVIRUS - CYTOKINE-DEPENDENT GROWTH
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DOI：
10.1247/csf.16.467
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发表时间：
1991-12-01
影响因子：
1.5
通讯作者：
SUDO, T
中科院分区：
文献类型：
--
作者：
OGAWA, Y;OHNO, N;SUDO, T
通过使用无辅助和复制缺陷的重组逆转录病毒编码SV40早期抗原（MV40），我们建立了连续的巨噬细胞（M-phi）系。所有系均为非产生型M-phi，具有不同的M-phi功能，如吞噬、细胞毒性、IL-1和TNF的产生。为了确定几种细胞因子对成熟M-phi细胞系生长的影响，我们在甲基纤维素培养中研究了这些已建立的M-phi细胞系对各种细胞因子的反应性。它们对几种细胞因子单独和联合的反应模式是不同的，这意味着可能存在成熟的M-phi亚群，其生长特征受几种细胞因子的调节。另一方面，所有的细胞系对白细胞介素-4 （IL-4）的反应都有效地产生了许多菌落。此外，IL-4与白细胞介素-3 （IL-3）共同促进了所有细胞系的集落形成。在几乎所有细胞系中，IL-4和巨噬细胞集落刺激因子（M-CSF）的联合作用也有类似的协同作用。新鲜巯基乙酸诱导的M-phi的菌落形成也得到了类似的结果。这些观察结果表明IL-4参与了成熟M-phi的生长。我们目前的结果表明，无辅助和复制缺陷的MV40可用于从原代M-phi获得连续和功能的细胞系。
By using a helper-free and replication-defective recombinant retrovirus encoding the SV40 early antigens (MV40), we have established continuous macrophage (M-phi) lines. All of the lines were nonproducer M phi's with differentiated M-phi functions such as phagocytosis, cytotoxicity, and IL-1 and TNF production. To determine the effects of several cytokines on growth of mature M-phi's, the responsiveness of these established M-phi lines to various cytokines was investigated in methylcellulose culture. Their response patterns to several cytokines alone and in combination were different, implying that there might be mature M-phi subpopulations with distinct growth profiles regulated by several cytokines. On the other hand, all of the lines efficiently yielded a number of colonies in response to interleukin-4 (IL-4) alone. Moreover, IL-4 cooperated with interleukin-3 (IL-3) to enhance colony formation of all the lines. A similarly synergistic effect was observed in combination of IL-4 and macrophage-colony stimulating factor (M-CSF) in almost all the lines. Similar results were obtained with colony formation of fresh thioglycolate-induced M-phi's. These observations suggested that IL-4 was involved in growth of mature M-phi's.Our present results suggest that the helper-free and replication-defective MV40 is of use to obtain continuous and functional cell lines from primary M-phi's.