Potential of SOX2 Inhibition for Embryonal Carcinoma
Potential of SOX2 Inhibition for Embryonal Carcinoma
复制标题
SOX2 抑制治疗胚胎癌的潜力
DOI：
10.1016/j.juro.2011.12.058
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Okamoto K.
中科院分区：
文献类型：
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作者：
Ushida H;Chano T;Minami K;Kita H;Kawakami T;Okabe H;Okada Y;Okamoto K.
目的一些非精原细胞肿瘤对任何类型的化疗都有抵抗力。控制胚胎癌细胞对于治疗非精原细胞肿瘤至关重要。我们在胚胎癌模型中建立了SOX2靶向治疗。材料和方法在一系列睾丸生殖细胞肿瘤组织样本中评估SOX2表达。利用胚胎癌模型在体外和体内分析了SOX2敲低的抗肿瘤作用。结果在睾丸生殖细胞肿瘤组织中，SOX2在胚胎癌病灶中表达，但在精原细胞瘤和卵黄囊肿瘤中表达阴性。在胚胎癌模型中，SOX2-siRNA 在体外诱导细胞凋亡，并在体内显着抑制生长。结论本研究显示了 SOX2 沉默对胚胎癌的治疗潜力。然而，SOX2-siRNA 向肿瘤的递送还需要进一步改进。
PurposeSome nonseminomatous germ cell tumors are resistant to any type of chemotherapy. Control of embryonal carcinoma cells is crucial to manage nonseminomatous germ cell tumors. We established SOX2 targeting therapy in an embryonal carcinoma model.Materials and MethodsSOX2 expression was evaluated in a series of testicular germ cell tumor tissue samples. The antitumor effect of SOX2 knockdown was analyzed in vitro and in vivo using an embryonal carcinoma model.ResultsIn testicular germ cell tumor tissue SOX2 was expressed in the foci of embryonal carcinoma but negative in seminoma and yolk sac tumors. In an embryonal carcinoma model SOX2-siRNA induced apoptotic cell death in vitro and significant growth suppression in vivo.ConclusionsThis study shows the therapeutic potential of SOX2 silencing for embryonal carcinoma. However, further improvements are needed in SOX2-siRNA delivery to the tumor.