Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids.
Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids.
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肝细胞球体的形成机制和超微结构评估。
DOI：
10.1007/bf02722946
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Hu,WS
中科院分区：
文献类型：
--
作者：
Peshwa,MV;Wu,FJ;Sharp,HL;Cerra,FB;Hu,WS
新鲜收获的大鼠肝细胞在未涂覆的带正电的聚苯乙烯表面形成球体。时间推移显微镜显示细胞运动和重组参与了球体的形成。扫描和透射电子显微镜的超微结构评估表明，细胞形态极化，球体内存在广泛的细胞间通讯。观察到胆小管结构围绕着每个单独的肝细胞，形成一个复杂的三维连续的通道网络，其末端似乎是球体表面的孔/孔。分化细胞形态的维持与肝细胞活性的保存和球体中组织特异性功能水平的提高相一致。
Freshly harvested rat hepatocytes form spheroids on uncoated positively charged polystyrene surfaces. Time lapse microscopy revealed that cell movement and reorganization were involved in spheroid formation. Ultrastructural evaluation using scanning and transmission electron microscopy indicated polarized cellular morphology and extensive cell-cell communication within spheroids. Bile canalicular structures were observed to surround each individual hepatocyte, forming an intricate three-dimensional continuous network of channels that appeared to end as pores/holes on the surface of the spheroid. The maintenance of differentiated cellular morphology coincided with preservation of hepatocyte viability and enhanced levels of tissue specific functions in spheroids.