Psoriasis patients generate increased serum levels of autoantibodies to tumor necrosis factor-α and interferon-α
Psoriasis patients generate increased serum levels of autoantibodies to tumor necrosis factor-α and interferon-α
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DOI：
10.1016/j.jdermsci.2009.08.006
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发表时间：
2009-12-01
影响因子：
4.6
通讯作者：
Karin, N.
中科院分区：
文献类型：
--
作者：
Bergman, R.;Ramon, M.;Karin, N.
背景：在扩展到人类的实验动物模型中显示，在自身免疫性疾病中，免疫系统对驱动疾病发病机制的有限数量的炎症介质产生有益的自身抗体（auto Ab）反应。目的：探讨银屑病患者自身抗体对细胞因子和趋化因子的影响。方法：采集牛皮癣（37例）、特应性皮炎（18例）和健康志愿者（56例）的血清。采用酶联免疫吸附法测定自身抗体（Abs）对tnf - α、干扰素- α (ifn - α)、白细胞介素-17 （IL-17）和趋化因子CCL2、CCL3和CCL5的滴度。采用体外功能测定法测定高效抗体对tnf - α和ifn - α的中和活性。结果：与健康受试者和AD患者相比，银屑病患者检测到自身抗体对tnf - α和ifn - α的滴度显著升高（平均滴度超过4倍）。这些自身抗体在体外表现出一定的中和活性，但它们的血清水平与疾病的强度和持续时间以及光疗诱导的缓解无关。在AD中检测到CCL3抗体的滴度显著增加，但程度较轻。结论：银屑病患者体内对两种关键细胞因子tnf - α和ifn - α的自身抗体水平明显升高。这些自身抗体在体外具有一定的中和活性，可能是一种附带现象，也可能在试图抑制正在进行的炎症过程中发挥作用。(C) 2009年由爱思唯尔爱尔兰有限公司代表日本皮肤病调查学会出版。
Background: It has been shown in experimental animal models that were extended to humans that during autoimmune conditions, the immune system generates beneficial autoantibody (auto Ab) response to a limited number of inflammatory mediators that drive the pathogenesis of the disease.Objective: To investigate the presence of auto Abs to cytokines and chemokines in psoriasis.Methods: Sera were obtained from patients with psoriasis (n = 37), atopic dermatitis (AD) (n = 18) and healthy Volunteers (n = 56). The titers of auto (Abs) to TNF-alpha, interferon-alpha (IFN-alpha), interleukin-17 (IL-17), and chemokines CCL2, CCL3 and CCL5 were determined using enzyme-linked immunosorbant assay. Neutralizing activities of high-titer auto Abs to TNF-alpha and IFN-alpha were determined using functional in vitro assays.Results: Highly significant increased titers of auto Abs to TNF-alpha and IFN-alpha were detected in patients with psoriasis compared with healthy subjects and patients with AD (mean titers more than fourfold). These auto Abs demonstrated some neutralizing activity in vitro, but their serum levels did not correlate with the intensity and duration of the disease and with phototherapy induced remissions. Significantly increased titers albeit to a lesser extent, of auto Abs to CCL3 were detected in AD.Conclusions: Psoriasis patients produce markedly increased levels of auto Abs to TNF-alpha and IFN-alpha which are two of the key cytokines in this disorder. The presence of these auto Abs which possess some neutralizing activity in vitro, may be an epiphenomenon or might play a role in attempting to suppress the ongoing inflammatory process. (C) 2009 Published by Elsevier Ireland Ltd on behalf of Japanese Society for investigative Dermatology.