Efficacy of Soap and Water and Alcohol-Based Hand-Rub Preparations against Live H1N1 Influenza Virus on the Hands of Human Volunteers
Efficacy of Soap and Water and Alcohol-Based Hand-Rub Preparations against Live H1N1 Influenza Virus on the Hands of Human Volunteers
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DOI：
10.1086/595845
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发表时间：
2009-02-01
影响因子：
11.8
通讯作者：
Birch, Christopher
中科院分区：
文献类型：
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作者：
Grayson, M. Lindsay;Melvani, Sharmila;Birch, Christopher
背景虽然已知大流行性流感和禽流感通过人手传播，但关于常规手卫生（HH）方案对大流行性流感和禽流感的有效性的数据很少。20名接种疫苗的抗体阳性医护人员的手被1 mL 10（7）组织培养感染剂量（TCID）（50）/0.1 mL活人甲型流感病毒污染（H1N1; A/New Caledonia/20/ 99），然后执行5项HH议定书中的1项（无HH [对照]，肥皂和水洗手[SW]，或使用3种含酒精的手擦之一[61.5%乙醇凝胶，70%乙醇加0.5%洗必泰溶液，或70%异丙醇加0.5%洗必泰溶液]）。通过病毒培养和实时逆转录酶聚合酶链反应（PCR）评估每次干预前后的H1N1浓度。H1N1在没有HH的情况下在手上自然存活160分钟也进行了评估。在短暂的皮肤空气干燥后，培养可检测和PCR可检测的H1N1立即减少-20名医护人员中有14名通过培养检测到H1N1（平均减少，10（3-4）TCID（50/）0.1 mL），而20名医护人员中有6名没有回收活的H1N1;所有20名医护人员的PCR检测结果都有类似的变化。根据培养结果，所有4种HH方案均显示出显著的抗病毒疗效（14/14例无可培养的H1N1; P
Background. Although pandemic and avian influenza are known to be transmitted via human hands, there are minimal data regarding the effectiveness of routine hand hygiene (HH) protocols against pandemic and avian influenza.Methods. Twenty vaccinated, antibody-positive health care workers had their hands contaminated with 1 mL of 10(7) tissue culture infectious dose (TCID)(50)/0.1 mL live human influenza A virus (H1N1; A/New Caledonia/20/ 99) before undertaking 1 of 5 HH protocols (no HH [control], soap and water hand washing [SW], or use of 1 of 3 alcohol-based hand rubs [61.5% ethanol gel, 70% ethanol plus 0.5% chlorhexidine solution, or 70% isopropanol plus 0.5% chlorhexidine solution]). H1N1 concentrations were assessed before and after each intervention by viral culture and real-time reverse-transcriptase polymerase chain reaction (PCR). The natural viability of H1N1 on hands for 160 min without HH was also assessed.Results. There was an immediate reduction in culture-detectable and PCR-detectable H1N1 after brief cutaneous air drying-14 of 20 health care workers had H1N1 detected by means of culture (mean reduction, 10(3-4) TCID(50/)0.1 mL), whereas 6 of 20 had no viable H1N1 recovered; all 20 health care workers had similar changes in PCR test results. Marked antiviral efficacy was noted for all 4 HH protocols, on the basis of culture results (14 of 14 had no culturable H1N1; P