An Adenosine Receptor for Olfaction in Fish
An Adenosine Receptor for Olfaction in Fish
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DOI：
10.1016/j.cub.2017.04.014
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发表时间：
2017-05-22
期刊：
影响因子：
9.2
通讯作者：
Yoshihara, Yoshihiro
中科院分区：
文献类型：
--
作者：
Wakisaka, Noriko;Miyasaka, Nobuhiko;Yoshihara, Yoshihiro
从食物源释放到环境水中的核苷酸被认为是许多鱼类的摄食线索。然而，鱼类如何敏感地检测到这些核苷酸仍然是未知的。在这里，我们发现了一种新的嗅觉机制，在斑马鱼的ATP传感器。在进入嗅鞘后，ATP通过在嗅上皮中表达的两种外核苷酸酶的酶促反应有效地转化为腺苷。腺苷随后激活一小群嗅觉感觉神经元，表达一种鱼类和两栖动物特有的新型腺苷受体A2c。然后，信息被传递到嗅球中的单个肾小球，并进一步传递到高级嗅觉中心的四个区域。这些结果提供了确凿的证据，一个复杂的酶联受体机制检测ATP作为一种食物来源的有吸引力的气味连接到觅食行为，是至关重要的和常见的水生低等脊椎动物。
Nucleotides released from food sources into environmental water are supposed to act as feeding cues for many fish species. However, it remains unknown how fish can sensitively detect those nucleotides. Here we discover a novel olfactory mechanism for ATP sensing in zebrafish. Upon entering into the nostril, ATP is efficiently converted into adenosine through enzymatic reactions of two ecto-nucleotidases expressed in the olfactory epithelium. Adenosine subsequently activates a small population of olfactory sensory neurons expressing a novel adenosine receptor A2c that is unique to fishes and amphibians. The information is then transmitted to a single glomerulus in the olfactory bulb and further to four regions in higher olfactory centers. These results provide conclusive evidence for a sophisticated enzyme-linked receptor mechanism underlying detection of ATP as a food-derived attractive odorant linking to foraging behavior that is crucial and common to aquatic lower vertebrates.