Characterization of a Borna disease virus field isolate which shows efficient viral propagation and transmissibility
Characterization of a Borna disease virus field isolate which shows efficient viral propagation and transmissibility
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DOI：
10.1016/j.micinf.2006.12.015
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发表时间：
2007-04-01
影响因子：
5.8
通讯作者：
Tomonaga, Keizo
中科院分区：
文献类型：
--
作者：
Watanabe, Yohei;Ibrahim, Madiha S.;Tomonaga, Keizo
为了调查博尔纳病病毒（BDV）的野外分离株的生物学特性，以及了解流行国家以外的BDV感染，我们从日本博尔纳病小母牛的脑样本中分离出病毒。我们证明，脑裂解物中含有BDV的复制产物，并诱导病毒在大鼠胶质瘤细胞中的繁殖，这表明在牛脑中存在有复制能力的BDV。与实验室适应的BDV毒株相比，Bo/04 w的BDV野外毒株显示出有效的病毒释放和传播性，并且在感染期间还显示出病毒磷蛋白（P）的独特表达模式。有趣的是，我们发现在Bo/04 w感染的细胞中P水平显著低，并且该分离物的转录比BDV实验室菌株的转录更有效。这些结果表明，田间分离物可以调节P在感染细胞中的表达在一个最佳水平。我们还证实了Bo/04 w在新生沙鼠脑中保持生物学意义。测序结果表明，尽管存在生物学差异，但田间分离株与BDV实验室菌株在遗传上密切相关。我们在这里讨论的序列相似性之间的BDV分离流行和非流行的国家。(c)2007年，Elsevier Masson SAS。All rights reserved.
To investigate the biological characteristics of field isolates of Borna disease virus (BDV), as well as to understand BDV infections outside endemic countries, we isolated the virus from brain samples of a heifer with Borna disease in Japan. We demonstrate that the brain lysate contained replication products of BDV and induced viral propagation in rat glioma cells, suggesting that a replication-competent BDV existed in the bovine brain. This field strain of BDV, named Bo/04w, showed efficient viral release and transmissibility and also displayed a distinct pattern of expression of viral phosphoprotein (P) during infection, as compared with laboratory-adapted BDV strains. Interestingly, we found the level of P to be significantly low in cells infected with Bo/04w, and the transcription of this isolate to be more efficient than that of laboratory strain of BDV. These results indicated that the field isolate may regulate the expression of P at an optimal level in infected cells. We also confirmed that Bo/04w maintains biological significance in neonatal gerbil brain. Sequencing revealed that despite the biological differences, the field isolate is closely related genetically to the laboratory strains of BDV. We discuss here the sequence similarities between BDV isolates from endemic and nonendemic countries. (c) 2007 Elsevier Masson SAS. All rights reserved.