[Effect of Doxorubicin on abcb4 Gene Expression in Zebrafish (Danio rerio) Embryos].
[Effect of Doxorubicin on abcb4 Gene Expression in Zebrafish (Danio rerio) Embryos].
复制标题
阿霉素对斑马鱼胚胎 abcb4 基因表达的影响
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
He Si-jia
中科院分区：
文献类型：
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作者：
Sun Cong-jie;He Zhi-xu;Shu Li-ping;Hu Rong-ying;He Si-jia
客观的
OBJECTIVE
To determine the effect of doxorubicin (DOX) on abcb4 gene expression and the role of abcb4 gene in multidrug-resistance.
METHODS
Zebrafish embryos were treated with 2 mL/L DMSO, 10 μmol/L DOX and 2 mL/L DMSO+10 μmol/L DOX, respectively. The zebrafish embryos treated with Eggwater served as controls. Exposures started at 4 to 16 cell stage of the embryos and terminated 120 hours post fertilization (hpf). The expression of abcb4 gene in zebrafish embryos was examined on 48, 72, 96, and 120 hpf with whole-mount in situ hybridization (WISH) and quantitative real-time PCR (qPCR).
RESULTS
Compared with the controls, DOX-exposed embryos had higher level of abcb4 gene expression (P < 0.05), but not for abcb5 gene. WISH showed that abcb4 gene was expressed in the guts of zebrafish embryos. However, those exposed to DOX also showed strong WISH signals in the brain and heart.
CONCLUSION
Doxorubicin increases the expression of abcb4 gene in zebrafish embryos. abcb4 gene may play an imoortant role in multidrug-resistance.