Application of high density steam flash-explosion in protein extraction of soybean meal
Application of high density steam flash-explosion in protein extraction of soybean meal
复制标题
高密度蒸汽闪爆在豆粕蛋白提取中的应用
DOI：
10.1016/j.jfoodeng.2012.12.006
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发表时间：
2013-05
影响因子：
5.5
通讯作者：
Zhang, Wenbin
中科院分区：
文献类型：
--
作者：
Yang, Ruijin;Zhao, Wei;Hua, Xiao;Zhang, Wenbin
采用高密度水蒸气闪爆技术(HDSFE)从豆粕中提取蛋白质。豆粕样品分别在1.3 Mpa和1.8 Mpa下处理60s、120s和180s。与未处理的豆粕相比，在1.8 Mpa压力下处理180 s后，蛋白质的浸提率从50.50%提高到65.66%。经HDSFE处理后的大豆分离蛋白(SPI)的乳化性能和脂肪吸附能力均比未处理的豆粕和白粉中的SPI有所提高。SPI的分子量分布分析表明，经HDSFE处理后，504 kDa和43.3 kDa左右的峰消失，669 kDa左右的峰增大，表明蛋白质聚集。凝胶电泳法显示，蛋白质通过共价键形成了高分子量的聚集体。
The high density steam flash-explosion (HDSFE) was used to extract protein from soybean meal. Soybean meal samples were treated at 1.3MPa and 1.8MPa for 60s, 120s and 180s, respectively. After HDSFE treatment at 1.8MPa for 180s, the extraction yield of protein was increased from 50.50% to 65.66% compared with untreated soybean meal. The emulsification properties and fat-binding capacity of soy protein isolate (SPI) extracted from soybean meal treated by HDSFE were all improved compared with SPI extracted from untreated soybean meal and white flakes. Molecular weight distribution analysis of SPI showed that after HDSFE treatment the peak with molecular weight about 504kDa and 43.3kDa disappeared and the peak with molecular weight about 669kDa increased indicating protein aggregation. Gel electrophoresis showed that high molecular weight aggregates of protein have been formed by covalent bond.
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DOI：
10.1007/s11746-004-0979-3
发表时间：
2004-08
期刊：
Journal of the American Oil Chemists' Society
影响因子：
--
作者：
H. Wang;L. Johnson
通讯作者：
H. Wang;L. Johnson
影响因子：
6
作者：
M. Negro;P. Manzanares;J. Oliva;I. Ballesteros;M. Ballesteros
通讯作者：
M. Negro;P. Manzanares;J. Oliva;I. Ballesteros;M. Ballesteros
影响因子：
5.5
作者：
Fuhrmeister, H;Meuser, F
通讯作者：
Meuser, F
影响因子：
6.1
作者：
PEARCE, KN;KINSELLA, JE
通讯作者：
KINSELLA, JE
DOI：
10.1021/bk-1998-0708.ch006
发表时间：
1998
期刊：
--
影响因子：
--
作者：
N. Hettiarachchy;U. Kalapathy
通讯作者：
N. Hettiarachchy;U. Kalapathy