Icariin, a natural flavonol glycoside, induces apoptosis in human hepatoma SMMC-7721 cells via a ROS/JNK-dependent mitochondrial pathway
Icariin, a natural flavonol glycoside, induces apoptosis in human hepatoma SMMC-7721 cells via a ROS/JNK-dependent mitochondrial pathway
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DOI：
10.1016/j.canlet.2010.07.009
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发表时间：
2010-12-08
期刊：
影响因子：
9.7
通讯作者：
Liu, Yingbin
中科院分区：
文献类型：
--
作者：
Li, Songgang;Dong, Ping;Liu, Yingbin
本研究探讨了淫羊藿苷对人肝癌SMMC-7721细胞的抗肿瘤作用及其机制。结果表明，淫羊藿苷通过增加线粒体膜电位、细胞色素c释放和caspase级联反应，激活c-Jun N-末端激酶（INK）磷酸化，从而激活线粒体/caspase凋亡通路。而p38、ERK 1/2和SP 600125则不表达此外，淫羊藿苷还能引起SMMC-7721细胞内活性氧（ROS）的产生，而抗氧化剂N-乙酰半胱氨酸几乎完全阻断了淫羊藿苷诱导的SMMC-7721细胞凋亡。诱导的JNK激活和细胞凋亡这些发现表明淫羊藿苷通过ROS/JNK依赖性线粒体途径诱导细胞凋亡（C）2010 Elsevier爱尔兰有限公司版权所有
In this study the anticancer effect of icariin a natural flavonol glycoside against human hepatoma SMMC-7721 cells and the underlying mechanisms were investigated Icariin triggered the mitochondrial/caspase apoptotic pathway indicated by enhanced Bax-to-Bcl-2 ratio loss of mitochodrial membrane potential cytochrome c release and caspase cascade Moreover icariin induced a sustained activation of the phosphorylation of c-Jun N-terminal kinase (INK) but not p38 and ERK1/2 and SP600125 (an inhibitor of JNK) almost reversed icariin-induced apoptosis in SMMC-7721 cells In addition icariin provoked the generation of reactive oxygen species (ROS) in SMMC-7721 cells while the antioxidant N-acetyl cysteine almost completely blocked icariin-induced JNK activation and apoptosis Taken together these findings suggest that icariin induces apoptosis through a ROS/JNK-dependent mitochondrial pathway (C) 2010 Elsevier Ireland Ltd All rights reserved