Spliceosomal protein U1A is involved in alternative splicing and salt stress tolerance in Arabidopsis thaliana
Spliceosomal protein U1A is involved in alternative splicing and salt stress tolerance in Arabidopsis thaliana
复制标题
剪接体蛋白 U1A 参与拟南芥的选择性剪接和盐胁迫耐受性。
DOI：
10.1093/nar/gkx1229
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发表时间：
2018-02-28
影响因子：
14.9
通讯作者：
Wang, Zhen-Yu
中科院分区：
文献类型：
--
作者：
Gu, Jinbao;Xia, Zhiqiang;Wang, Zhen-Yu
土壤盐碱化是全球可持续农业生产的重大威胁。植物必须调整其发育和生理过程以科普盐胁迫。虽然适应的能力最终取决于基因组，剪接体介导的选择性剪接（AS）提供的基因调控的特殊的多功能性是必不可少的，在这些适应过程。然而，剪接体在植物胁迫反应中的功能知之甚少。在这里，我们报告的深入表征的U1剪接体蛋白，AtU 1A，在控制AS的前mRNA盐胁迫和盐胁迫耐受拟南芥。atu 1a突变体对盐胁迫非常敏感，在盐胁迫下积累了比野生型更多的活性氧。RNA-seq分析表明AtU 1A可能通过调节5'剪接位点的识别来调控许多基因的AS。我们发现AtU 1A与ROS解毒相关基因ACO 1的前体mRNA相关，并且是调节ACO 1 AS所必需的。ACO 1对于盐耐受性是重要的，因为ACO 1在atu 1a突变体中的异位表达可以部分地挽救其盐过敏表型。我们的研究结果强调了AtU 1A作为植物前mRNA加工和耐盐性调节剂的关键作用。
Soil salinity is a significant threat to sustainable agricultural production worldwide. Plants must adjust their developmental and physiological processes to cope with salt stress. Although the capacity for adaptation ultimately depends on the genome, the exceptional versatility in gene regulation provided by the spliceosome-mediated alternative splicing (AS) is essential in these adaptive processes. However, the functions of the spliceosome in plant stress responses are poorly understood. Here, we report the in-depth characterization of a U1 spliceosomal protein, AtU1A, in controlling AS of pre-mRNAs under salt stress and salt stress tolerance in Arabidopsis thaliana. The atu1a mutant was hypersensitive to salt stress and accumulated more reactive oxygen species (ROS) than the wild-type under salt stress. RNA-seq analysis revealed that AtU1A regulates AS of many genes, presumably through modulating recognition of 5' splice sites. We showed that AtU1A is associated with the pre-mRNA of the ROS detoxification-related gene ACO1 and is necessary for the regulation of ACO1 AS. ACO1 is important for salt tolerance because ectopic expression of ACO1 in the atu1a mutant can partially rescue its salt hypersensitive phenotype. Our findings highlight the critical role of AtU1A as a regulator of pre-mRNA processing and salt tolerance in plants.