A plant plasma membrane ATP binding cassette-type transporter is involved in antifungal terpenoid secretion
A plant plasma membrane ATP binding cassette-type transporter is involved in antifungal terpenoid secretion
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DOI：
10.1105/tpc.13.5.1095
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发表时间：
2001-05-01
期刊：
影响因子：
11.6
通讯作者：
Boutry, M
中科院分区：
文献类型：
--
作者：
Jasinski, M;Stukkens, Y;Boutry, M
三磷酸腺苷结合盒(ABC)转运体在所有物种中都存在，主要是因为它们具有传递耐药性的能力。到目前为止，大多数以植物为特征的ABC转运蛋白都定位在液泡膜上，并被认为参与了细胞毒素的细胞内隔离。基于某些ABC转运蛋白可能参与防御代谢产物的分泌及其表达可能受这些代谢物浓度调节的假设，我们用Sclareolide处理白叶烟草细胞培养物，Sclareolide与Sclareol相似，Sclareol是一种在烟草叶表面产生的抗真菌二萜，这导致了一个160kD的质膜蛋白的出现，该蛋白被部分测序。克隆了相应的基因(NpABC1)，编码ABC转运蛋白，体外和原位免疫检测表明NpABC1定位于细胞膜，在正常情况下，NpABC1在叶表皮、细胞培养和叶组织中都有表达，Sclareolide和Sclareol对NpABC1的表达有明显的促进作用。在NpABC1诱导的同时，细胞获得了分泌标记的合成巩膜内酯衍生物的能力。这些数据表明，NpABC1参与了一种次生代谢物的分泌，该次生代谢物在植物防御中发挥作用。
ATP binding cassette (ABC) transporters, which are found in all species, are known mainly for their ability to confer drug resistance. To date, most of the ABC transporters characterized in plants have been localized in the vacuolar membrane and are considered to be involved in the intracellular sequestration of cytotoxins. Working on the assumption that certain ABC transporters might be involved in defense metabolite secretion and their expression might be regulated by the concentration of these metabolites, we treated a Nicotiana plumbaginifolia cell culture with sclareolide, a close analog of sclareol, an antifungal diterpene produced at the leaf surface of Nicotiana spp; this resulted in the appearance of a 160-kD plasma membrane protein, which was partially sequenced. The corresponding cDNA (NpABC1) was cloned and shown to encode an ABC transporter, In vitro and in situ immunodetection showed NpABC1 to be localized in the plasma membrane, Under normal conditions, expression was found in the leaf epidermis, In cell culture and in leaf tissues, NpABC1 expression was strongly enhanced by sclareolide and sclareol. In parallel with NpABC1 induction, cells acquired the ability to excrete a labeled synthetic sclareolide derivative. These data suggest that NpABC1 is involved in the secretion of a secondary metabolite that plays a role in plant defense.