Effects of temperature and glycerol on the resonance Raman spectra of cytochrome c peroxidase and selected mutants.
Effects of temperature and glycerol on the resonance Raman spectra of cytochrome c peroxidase and selected mutants.
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温度和甘油对细胞色素 c 过氧化物酶和选定突变体的共振拉曼光谱的影响。
DOI：
10.1021/bi00438a024
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
J. Mauro
中科院分区：
文献类型：
--
作者：
G. Smulevich;A. Mantini;A. English;J. Mauro
在295 K和10 K下，记录了Fe Ⅲ酵母天然细胞色素c过氧化物酶（CCP）及其5个突变体[CCP（MI），Phe-51，Leu-48，Lys-48，Asn-235和Phe-191]在磷酸盐缓冲液（pH 7.0）和甘油/磷酸盐混合物中的高频共振拉曼光谱。甘油在室温下诱导一些CCP突变体的血红素配位变化。它明显地削弱了铁原子与远端血红素腔中的配体的结合，并将血红素推向5-配位的高自旋状态。在10 K时，天然CCP和所有突变体（Phe-51仍为6配位、高自旋）的自旋和配位态分布与295 K时不同。在pH 7的磷酸盐缓冲液中冷却后，以及在66%甘油/磷酸盐中冷却到更小的程度时，内部强场配体与Fe配位。一个可能的候选者是H2O-595，它可以成为氢键和/或质子转移到H2O-648和/或远端His-52上的强场配体。然而，不能排除远端His-52本身作为配位配体，考虑到在室温下结合H2O-595的Phe-51突变体在10 K下不像其他突变体那样显示大的6配位、低自旋组分。这些结果清楚地表明，CCP及其突变体中的Fe配位对温度和溶剂组成都很敏感。
The high-frequency resonance Raman spectra of FeIII yeast native cytochrome c peroxidase (CCP) and five of its mutants [CCP(MI), Phe-51, Leu-48, Lys-48, Asn-235, and Phe-191] were recorded in phosphate buffer, pH 7.0, and in glycerol/phosphate mixtures at 295 and 10 K. Glycerol induces heme coordination changes in some of the CCP mutants at room temperature. It apparently weakens the binding of the Fe atom to ligands in the distal heme cavity and drives the heme toward the 5-coordinate, high-spin state. At 10 K, native CCP and all the mutants (except Phe-51 which remains 6-coordinate, high-spin) show various distributions of spin and coordination states which differ from those observed at 295 K. Upon cooling in phosphate buffer, pH 7, and to a much lesser extent in 66% glycerol/phosphate, an internal strong-field ligand is coordinated to the Fe. A likely candidate is H2O-595, which could become a strong-field ligand on H-bonding and/or proton transfer to H2O-648, and/or the distal His-52. However, distal His-52 itself cannot be ruled out as the coordinating ligand considering that the Phe-51 mutant, which binds H2O-595 at room temperature, does not show a large 6-coordinate, low-spin component at 10 K like the other mutants. These results clearly indicate that the Fe coordination in CCP and its mutants is sensitive to both temperature and solvent composition.
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不同构象状态下细胞色素 C 过氧化物酶-一氧化碳加合物的拉曼光谱和红外光谱。
影响因子：
2.9
作者：
Smulevich,G;Evangelista-Kirkup,R;English,A;Spiro,TG
通讯作者：
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DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Hori,H;Yonetani,T
通讯作者：
Yonetani,T
影响因子：
2.9
作者：
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通讯作者：
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影响因子：
2.9
作者：
Andersson,LA;Renganathan,V;Loehr,TM;Gold,MH
通讯作者：
Gold,MH