A COMPENSATORY BASE CHANGE IN U1 SNRNA SUPPRESSES A 5' SPLICE SITE MUTATION
A COMPENSATORY BASE CHANGE IN U1 SNRNA SUPPRESSES A 5' SPLICE SITE MUTATION
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DOI：
10.1016/0092-8674(86)90064-4
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发表时间：
1986-09-12
期刊：
影响因子：
64.5
通讯作者：
WEINER, AM
中科院分区：
文献类型：
--
作者：
ZHUANG, Y;WEINER, AM
间接证据表明，U1 snRNA的5“末端通过互补碱基配对识别mRNA前体中的5”剪接位点。为了验证这一假设，我们询问腺病毒E1A基因的12S和13S 5“剪接位点的点突变是否可以被人U1 snRNA中的补偿性碱基变化抑制。当突变体E1A和U1基因共转染到HeLa细胞中时，我们观察到12S剪接位点+5位的一个突变的有效抑制，但13S剪接位点+3位的另一个突变的抑制非常弱。这些和其他结果表明，U1和5“剪接位点之间的碱基配对是必要的，但不足以剪接mRNA前体。
Indirect evidence suggests that the 5'' end of U1 snRNA recognizes the 5'' splice site in mRNA precursors by complementary base pairing. To test this hypothesis, we asked whether point mutations in the alternative 12S and 13S 5'' splice sites of the adenovirus E1A gene can be suppressed by compensatory base changes in human U1 snRNA. When the mutant E1A and U1 genes are cotransfected into HeLa cells, we observe efficient suppression of one mutation at position +5 in the 12S splice site, but exceedingly weak suppression of another mutation at position +3 in the 13S splice site. These and other results suggest that base pairing between U1 and the 5'' splice site is necessary but not sufficient for the splicing of mRNA precursors.