Involvement of Membrane Progestin Receptor Beta (mPRβ/Paqr8) in Sex Pheromone Progestin-Induced Expression of Luteinizing Hormone in the Pituitary of Male Chinese Black Sleeper (Bostrychus Sinensis).
Involvement of Membrane Progestin Receptor Beta (mPRβ/Paqr8) in Sex Pheromone Progestin-Induced Expression of Luteinizing Hormone in the Pituitary of Male Chinese Black Sleeper (Bostrychus Sinensis).
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膜孕激素受体β (mPR beta/Paqr8) 参与性信息素孕激素诱导的中国雄性黑睡鱼 (Bostrychus Sinensis) 垂体中黄体生成素的表达
DOI：
10.3389/fendo.2018.00397
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发表时间：
2018
影响因子：
5.2
通讯作者：
Chen SX
中科院分区：
文献类型：
--
作者：
Zhang YT;Hong WS;Liu DT;Qiu HT;Zhu Y;Chen SX
本实验室前期研究表明，17α，20β-二羟基-4-异戊烯-3-酮（DHP）作为性信息素诱导中华长蠹（Bostrychus sinensis）繁殖成功，但其作用机制尚不清楚。在本研究中，我们克隆了促性腺激素亚基（cgα，fshβ和lhβ）的cDNA，并发现，在暴露于5 nM DHP后，lhβ的转录水平显着增加，在暴露后6 h的垂体;血浆11-KT水平增加，在暴露后24 h的成熟雄鱼。相反，DHP暴露未能增加未成熟雄性鱼脑垂体中lhβ的转录水平，表明对DHP的反应取决于生殖状态。有趣的是，在减数分裂后期，在共同注射人绒毛膜促性腺激素（HCG）和促黄体生成素释放酶-A3（LHRH-A3）后，在雄性鱼的嗅玫瑰花结中，除了其他的孕酮受体没有观察到增加外，还观察到孕酮受体膜组分2（progesterone receptor membrane component 2，paqr 8，也称为mPRβ）和孕酮受体膜组分2（progesterone receptor membrane component 2）的表达显著增加。此外，Paqr 8蛋白定位于嗅感觉神经元的树突状突起，其在体内暴露于DHP后被激活。核孕酮受体拮抗剂RU 486不抑制DHP诱导的垂体lhβ表达。以上结果表明，性信息素DHP可提高成熟雄蛾垂体lhβ转录本的表达和血浆11-KT水平，对雄蛾的繁殖具有重要作用; Paqr 8可能参与了雄蛾B嗅丛对性信息素DHP的应答。中国的。
Our previous studies showed that 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) acted as a sex pheromone to induce reproductive success in Chinese black sleeper (Bostrychus sinensis), but its functional mechanism remains unclear. In the present study, we cloned the cDNAs of the gonadotropin subunits (cgα, fshβ, and lhβ), and found that, in exposure to 5 nM DHP, transcript levels of lhβ significantly increased in the pituitary at 6 h post exposure; plasma 11-KT levels increased at 24 h post exposure in mature male fish. In contrast, DHP exposure failed to increase the transcript levels of lhβ in the pituitary of immature male fish, suggesting that the responsiveness to DHP depends on reproductive status. Interestingly, expression of progestin and adipoQ receptor 8 (paqr8, also known as mPRβ) and progesterone receptor membrane component 2 significantly increased in the olfactory rosette of male fish at late meiosis stage following a co-injection of human chorionic gonadotropin (HCG) and luteinizing hormone releasing hormone-A3 (LHRH-A3), while no increases of other progestin receptors were observed. Moreover, Paqr8 protein was localized in the dendritic knobs of the olfactory sensory neurons, which were activated following the in vivo exposure to DHP. The DHP-induced expression of lhβ in pituitary was not inhibited by RU486, an antagonist of nuclear progesterone receptor. Taken together, our results suggested that sex pheromone DHP increased the expression of lhβ transcript in the pituitary and plasma 11-KT levels of mature male, important for reproduction; and Paqr8 might be involved in responding to sex pheromone DHP in the olfactory rosette of male B. sinensis.