Phage-induced lysis enhances biofilm formation in Shewanella oneidensis MR-1
Phage-induced lysis enhances biofilm formation in Shewanella oneidensis MR-1
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DOI：
10.1038/ismej.2010.153
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发表时间：
2011-04-01
期刊：
影响因子：
11
通讯作者：
Thormann, Kai M.
中科院分区：
文献类型：
--
作者：
Goedeke, Julia;Paul, Kristina;Thormann, Kai M.
希瓦氏菌MR-1能够形成高度结构化的表面附着群落。通过DNA酶I处理，我们证明了细胞外DNA（eDNA）作为一个结构组成部分，在所有阶段的生物膜形成在静态和流体动力学条件下。我们确定了eDNA是否是通过S基因组中的三个前噬菌体LambdaSo、MuSo 1和MuSo 2介导的细胞裂解释放的。oneidensis MR-1。突变体分析和感染研究表明，所有三种前噬菌体都可能单独导致细胞溶解。然而，只有LambdaSo和MuSo 2形成感染性噬菌体颗粒。在静态孵育的早期阶段已经发生噬菌体释放和细胞裂解。缺乏原噬菌体的突变体在纯培养中明显不容易裂解。此外，噬菌体少突变体在生物膜形成的所有发展阶段严重受损，三维生长独立于eDNA作为结构组分而发生。因此，我们建议在S. oneidensis MR-1原噬菌体介导的裂解导致关键的生物膜促进因子，特别是eDNA的释放。The ISME Journal（2011）5，613-626; doi：10.1038/ismej.2010.153; 2010年10月21日在线发表
Shewanella oneidensis MR-1 is capable of forming highly structured surface-attached communities. By DNase I treatment, we demonstrated that extracellular DNA (eDNA) serves as a structural component in all stages of biofilm formation under static and hydrodynamic conditions. We determined whether eDNA is released through cell lysis mediated by the three prophages LambdaSo, MuSo1 and MuSo2 that are harbored in the genome of S. oneidensis MR-1. Mutant analyses and infection studies revealed that all three prophages may individually lead to cell lysis. However, only LambdaSo and MuSo2 form infectious phage particles. Phage release and cell lysis already occur during early stages of static incubation. A mutant devoid of the prophages was significantly less prone to lysis in pure culture. In addition, the phage-less mutant was severely impaired in biofilm formation through all stages of development, and three-dimensional growth occurred independently of eDNA as a structural component. Thus, we suggest that in S. oneidensis MR-1 prophage-mediated lysis results in the release of crucial biofilm-promoting factors, in particular eDNA. The ISME Journal (2011) 5, 613-626; doi:10.1038/ismej.2010.153; published online 21 October 2010