Identification and Blood Transfusion of Rare B(A) Blood Group.
Identification and Blood Transfusion of Rare B(A) Blood Group.
复制标题
稀有 B(A) 血型的鉴定和输血。
DOI：
10.7754/clin.lab.2020.201203
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发表时间：
2021
影响因子：
0.7
通讯作者：
Haitao Huang
中科院分区：
文献类型：
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作者：
Yongqiang Shan;Jinghui Chong;Chunhong Du;Yaohua Xu;Haitao Huang
背景
目的研究天津地区一例B（A）血型患者的血清学和基因特征。目的探讨B（A）血型患者的输血情况。
方法
对天津医科大学总医院1例ABO分型不一致的患者，采用标准血清学技术进行ABO亚群鉴定。从他的样本中收集DNA（脱氧核糖核酸），并使用PCR（聚合酶链反应）对外显子6和7进行测序。对PCR产物进行测序，以确定ABO亚型和B（A）等位基因。通过ABO基因外显子6和7的DNA测序进行ABO基因的分子基因分型。
结果
患者红细胞与抗-A呈混合凝集反应（MF），与抗-H呈弱反应（W+）。但与抗B有较强的凝集反应。患者血清中存在抗A抗体，但不与B细胞反应。患者红细胞的血清学特征与AxB亚型相似。ABO基因第6、7外显子的261 delG、297 A>G、526 C>G、640 A>G、657 C>T、703 G>A、796 C>A、803 G>C、930 G>A位点存在变异。该序列与B101相似，除了nt 640（A>G）。基因分型结果表明该标本为B（A）04。
结论
分子基因分型证实ABO状态为B（A）04。B（A）血型与复杂的血清学表型相关，DNA检测对于这种非典型表型样本是必要的。为确保输血安全，本研究制定了B（A）型患者紧急输血程序。
BACKGROUND
To study serologic and gene characteristics of the B(A) blood group of one patient in Tianjin, China. To investigate the blood transfusion for patients with B(A) blood group.
METHODS
ABO subgroups were identified by standard serologic techniques in ABO typing discrepancy sample from one patient at the Tianjin Medical University General Hospital. DNA (deoxyribonucleic acid) was collected from his sample and PCR (polymerase chain reaction) was used to sequence exons 6 and 7. PCR products were sequenced to identify ABO subgroups and the B(A) allele. Molecular genotyping of the ABO gene was performed by DNA sequencing of exons 6 and 7 of the ABO gene.
RESULTS
The patient's red cells showed mixed field agglutination reaction (MF) with anti-A and weak reaction (W+) with anti-H. However, it has strong agglutination reaction with anti-B. The patient's serum showed the presence of anti-A antibody, while it can't react with B cell. The serological characteristics of the patient's red cells were similar to AxB subtype. Variation sites were confirmed at 261delG, 297 A>G, 526 C>G, 640 A>G, 657 C>T, 703 G>A, 796 C>A, 803 G>C, 930 G>A in exon 6 and 7 of the ABO gene. The sequence was similar to B101 except for nt640 (A>G). Genotyping indicated that the specimen was B(A)04.
CONCLUSIONS
Molecular genotyping confirmed the ABO status as B(A)04. The B(A) blood group is associated with a complicated serologic phenotype and DNA detection is necessary for this atypical phenotype sample. To ensure the safety of transfusion, this study developed an emergency transfusion procedure for patients with B(A) type.