Detection of microfilariae with counting chamber technique in some Nigerian rural communities
Detection of microfilariae with counting chamber technique in some Nigerian rural communities
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在尼日利亚一些农村社区采用计数室技术检测微丝蚴
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
D. Akinboye
中科院分区：
文献类型：
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作者：
O. Agbolade;D. Akinboye
传统的染色厚涂片技术（TS）有低估微丝蚴血症者微丝蚴（mf）的固有可能性。计数室（CC）技术在检测MF的可用性，确定在尼日利亚的三个农村社区，Loa loa和持久性曼森氏菌是地方病。于1996年2月至1998年7月间，采用手指针刺法采集612例受试者（男性334例，女性278例）的血液样本。使用TS或/和CC技术检查每份血样的mf。CC技术的敏感性（79.3%）高于TS技术（39.1%）。对于这两种技术，微丝蚴范围为1至20 mf/50 μl血液。两种技术的微丝蚴几何平均强度之间没有统计学显著差异。《非洲生物技术杂志》第4卷（4），pp. 367-370，2005年
The conventional stained thick smear technique (TS) has innate possibility of underestimation of microfilariae (mf) in microfilaraemic subjects. The usability of counting chamber (CC) technique in the detection of mf was determined in three Nigerian rural communities where Loa loa and Mansonella perstans are endemic. Blood samples were collected by finger-pricking method from 612 subjects (334 males, 278 females), between February 1996 and July 1998. Each blood sample was examined for mf using TS or/and CC technique(s). CC technique had statistically higher sensitivity (79.3%) than TS technique (39.1%). For both techniques, the microfilarial range was 1 to 20 mf/50 μl of blood. There was no statistically significant differences between the microfilarial geometric mean intensities with both techniques. African Journal of Biotechnology Vol. 4 (4), pp. 367-370, 2005