A chronic model for evaluating the itching associated with allergic conjunctivitis in rats
A chronic model for evaluating the itching associated with allergic conjunctivitis in rats
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DOI：
10.1016/j.intimp.2003.10.013
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发表时间：
2004-01-01
影响因子：
5.6
通讯作者：
Kamei, C
中科院分区：
文献类型：
--
作者：
Minami, K;Kamei, C
本研究的目的是开发一种新模型来评估与大鼠过敏性结膜炎相关的瘙痒。重复局部施用抗原导致致敏动物的抓眼行为增加，并且在第21天至42天观察到显着差异。在过敏症状、充血和水肿方面观察到几乎相同的结果。滴注组胺也会导致抓眼行为增加。致敏后 42 天局部使用抗原可增加抓眼行为中组胺的敏感性。此外，在致敏大鼠中，从第21天到第42天重复局部施用抗原，结膜嗜酸性粒细胞的数量显着增加。一些抗过敏药物，如奥洛他定（H-1拮抗剂）、西替啶（H-1拮抗剂）和雷马曲班（血栓烷A（2）（TXA（2））拮抗剂）以剂量相关的方式抑制局部致敏引起的抓眼行为。然而，扎鲁司特（cys-LT拮抗剂）即使在30 mg/kg的剂量下也没有引起明显的抑制作用。目前过敏性结膜炎瘙痒模型的发现主要是通过组胺H-1活性，血栓素A（2）受体也参与了反应。 (C) 2004 Elsevier B.V. 保留所有权利。
The present study was performed to develop a new model for evaluating itching associated with allergic conjunctivitis in rats. Repeated topical application of antigen caused an increase in eye scratching behavior in sensitized animals, and a significant difference was observed from days 21 to 42. Almost the same findings were observed in allergic symptoms, hyperemia and edema. Instillation of histamine also resulted in an increase in eye scratching behavior. The sensitivity to histamine in eye scratching behavior was increased by topical antigen application for 42 days after sensitization. In addition, the number of conjunctival eosinophils was significantly increased by repeated topical antigen application from days 21 to 42 in sensitized rats. Some anti-allergic drugs such as olopatadine (H-1 antagonist), cetiridine (H-1 antagonist) and ramatroban (thromboxane A(2) (TXA(2)) antagonist) caused an inhibition of eye scratching behavior induced by topical sensitization in a dose-related manner. However, zafirlukast (cys-LT antagonist) caused no significant inhibition even at a dose of 30 mg/kg.The findings in present model of itching in allergic conjunctivitis were mainly through histamine H-1-activity, and thromboxane A(2) receptors were also involved in the response. (C) 2004 Elsevier B.V. All rights reserved.