Identification, expression and functional analysis of U3 snoRNA genes from Neurospora crassa
Identification, expression and functional analysis of U3 snoRNA genes from Neurospora crassa
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粗糙脉孢菌U3 snoRNA基因的鉴定、表达及功能分析
DOI：
10.1016/j.pnsc.2008.04.017
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发表时间：
2009-02-10
影响因子：
4.7
通讯作者：
Qu, Lianghu
中科院分区：
文献类型：
--
作者：
Liu, Na;Huang, Qiaojuan;Qu, Lianghu
通过对粗糙脉孢菌小分子RNA构建的cDNA文库的筛选和基因组分析，鉴定出3个长度分别为262、270和275 nt的U3 snoRNA，分别命名为NcU 3A、NcU 3B和NcU 3C。通过北方印迹杂交进一步分析三种U3 snoRNA的表达。同时，还鉴定了两个不同大小的5 '截短的U3转录物，表明U3 snoRNA的复杂转录后加工。除了已知的18 S rRNA功能元件外，还在NcU 3A中发现了一个新的26 S rRNA反义序列。引物延伸分析表明，该序列不指导26 S rRNA的2 '-O-核糖甲基化，但可能起rRNA伴侣的作用。在N. crassa的三个U3 snoRNA基因是独立转录的，每个基因都含有一个小的内含子。在snoRNA基因中存在相同的内含子插入位点。crassa和酵母显示了这些生物中内含子的古老起源。我们的研究结果为U3 snoRNA家族的结构和功能多样性提供了新的信息。(C)2008年国家自然科学基金、中国科学院。出版社：Elsevier Limited，Science China Press All rights reserved.
By screening of a cDNA library constructed with small RNAs from Neurospora crassa and genome analysis, three U3 snoRNAs 262, 270 and 275 nt were identified and named as NcU3A, NcU3B and NcU3C, respectively. Expression of the three U3 snoRNA was further analyzed by Northern blot hybridization. Meanwhile, two 5'-truncated U3 transcripts with different sizes were also identified, indicating a complex posttranscriptional processing of the U3 snoRNAs. In addition to the known functional elements to 18S rRNA, a novel antisense sequence to 26S rRNA was found in NcU3A. A primer extension assay showed that the sequence did not guide 2'-O-ribose methylation of 26S rRNA but probably functioned as an rRNA chaperon. In the N. crassa three U3 snoRNA genes are independently transcribed and each of them contains a small intron. The same intron-inserting site in the snoRNA genes among N. crassa and yeasts shows the ancient origin of the intron in these organisms. Our results provide new into the structural and functional diversity of U3 snoRNA family. (C) 2008 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science China Press. All rights reserved.