Exposure of human vascular endothelial cells to sustained hydrostatic pressure stimulates proliferation -: Involvement of the αv integrins
Exposure of human vascular endothelial cells to sustained hydrostatic pressure stimulates proliferation -: Involvement of the αv integrins
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DOI：
10.1161/01.res.84.3.315
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发表时间：
1999-02-19
影响因子：
20.1
通讯作者：
Gerritsen, ME
中科院分区：
文献类型：
--
作者：
Schwartz, EA;Bizios, R;Gerritsen, ME
本研究探讨了持续静水压力（SHP;高达4厘米水柱）对人脐静脉内皮细胞（HUVEC）增殖，粘着斑（FAP）组织，整合素表达的影响。HUVEC暴露于SHP刺激细胞增殖和整合素亚基α（v）表达的选择性增加。早在暴露于压力后4小时就观察到α（v）的增加，并且在溴脱氧尿苷标记指数可检测到增加之前。激光共聚焦显微镜研究证明了α（V）整合素与FAP的共定位。压力处理的细胞中的单个FAP表现出减小的面积和增加的纵横比，并且定位于细胞的外周和更中心的区域，与在控制压力条件下维持的细胞中的细胞外周的偏好相反。压力诱导的α（V）分布的变化对细胞具有功能性后果：细胞对玻连蛋白的粘附性增加，α（V）拮抗剂阻断压力诱导的增殖反应。因此，本研究表明α（V）整合素在内皮细胞的压力机械转导中的作用。
The present study investigated the effects of sustained hydrostatic pressure (SHP; up to 4 cm H2O) on human umbilical vein endothelial cell (HUVEC) proliferation, focal adhesion plaque (FAP) organization, and integrin expression. Exposure of HUVECs to SHP stimulated cell proliferation and a selective increase in the expression of integrin subunit alpha(v). The increase in alpha(v) was observed as early as 4 hours after exposure to pressure and preceded detectable increases in the bromodeoxyuridine labeling index. Laser confocal microscopy studies demonstrated colocalization of the alpha(v) integrin to FAPs. The individual FAPs in pressure-treated cells demonstrated a reduced area and increased aspect ratio and were localized to both peripheral and more central regions of the cells, in contrast to the predilection for the cell periphery in cells maintained under control pressure conditions, The pressure-induced changes in alpha(v) distribution had functional consequences on the cells: adhesivity of the cells to vitronectin was increased, and alpha(v) antagonists blocked the pressure-induced proliferative response, Thus, the present study suggests a role for alpha(v) integrins in the mechanotransduction of pressure by endothelial cells.