Conditioned Media from Mesenchymal Stem Cells Enhanced Bone Regeneration in Rat Calvarial Bone Defects
Conditioned Media from Mesenchymal Stem Cells Enhanced Bone Regeneration in Rat Calvarial Bone Defects
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DOI：
10.1089/ten.tea.2011.0325
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发表时间：
2012-07-01
影响因子：
4.1
通讯作者：
Ueda, Minoru
中科院分区：
文献类型：
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作者：
Osugi, Masashi;Katagiri, Wataru;Ueda, Minoru
组织工程最近成为一种可用于骨增强的治疗方法。然而，这种方法有几个问题，如高资本投资和昂贵的细胞培养，关于细胞处理的复杂安全和质量管理问题，以及侵入性细胞收集过程中的患者问题。此外，据报道，干细胞在培养过程中会分泌许多生长因子和趋化因子，这些因子会影响细胞的特性和行为。本研究探讨了干细胞培养条件培养基对骨再生的影响。人骨髓间充质干细胞（MSC-CM）培养的条件培养基增强了大鼠骨髓间充质干细胞（rMSCs）的迁移、增殖和成骨标志基因（如骨钙素和Runx2）的表达。MSC-CM包括胰岛素样生长因子-1、血管内皮生长因子等细胞因子。在体内，采用人MSCs/琼脂糖（MSCs）、MSC-CM/琼脂糖（MSC-CM）、Dulbecco改良Eagle培养基(不含血清[DMEM(-)]/琼脂糖[DMEM（-)]、PBS/琼脂糖（PBS）和仅缺损（defect））中的一种材料，将制备好的大鼠颅骨骨缺损模型植入5个不同的大鼠组。4周和8周后，使用显微计算机断层扫描（micro-CT）和组织学分析对种植体切片进行评估。Micro-CT分析显示，与其他组相比，MSC-CM组新生骨面积更大(p
Tissue engineering has recently become available as a treatment procedure for bone augmentation. However, this procedure has several problems, such as high capital investment and expensive cell culture, complicated safety and quality management issues regarding cell handling, and patient problems with the invasive procedure of cell collection. Moreover, it was reported that stem cells secrete many growth factors and chemokines during their cultivation, which could affect cellular characteristics and behavior. This study investigated the effect of stem-cell-cultured conditioned media on bone regeneration. Cultured conditioned media from human bone marrow-derived mesenchymal stem cells (MSC-CM) enhanced the migration, proliferation, and expression of osteogenic marker genes, such as osteocalcin and Runx2, of rat MSCs (rMSCs) in vitro. MSC-CM includes cytokines such as insulin-like growth factor-1 and vascular endothelial growth factor. In vivo, a prepared bone defect of a rat calvarial model was implanted in five different rat groups using one of the following graft materials: human MSCs/agarose (MSCs), MSC-CM/agarose (MSC-CM), Dulbecco's modified Eagle's medium without serum [DMEM(-)]/agarose [DMEM(-)], PBS/agarose (PBS), and defect only (Defect). After 4 and 8 weeks, implant sections were evaluated using microcomputed tomography (micro-CT) and histological analysis. Micro-CT analysis indicated that the MSC-CM group had a greater area of newly regenerated bone compared with the other groups (p