In vivo and in vitro stability of modified poly(urethaneurea) blood sacs
In vivo and in vitro stability of modified poly(urethaneurea) blood sacs
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DOI：
10.1106/r01n-dt0c-uu1c-2gm8
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发表时间：
2000-04-01
影响因子：
2.9
通讯作者：
Werley, T
中科院分区：
文献类型：
--
作者：
Liu, Q;Runt, J;Werley, T
在本研究中，我们研究了改性聚（脲）（BioSpan MS/0.4）血囊的体内和体外稳定性。血囊主要用于植入小牛体内5 - 160天的左心室辅助装置。还对类似囊进行了体外循环试验。采用各种分析方法来表征体内或体外服务后的囊和相应的保留的“对照”囊。这些方法包括ATR-FTIR光谱，扫描电子显微镜和凝胶渗透色谱。一般而言，植入和体外循环的囊的特征与其对照囊相似。测试后，热性能和微拉伸性能没有变化。ATR-FTIR光谱也是如此，表明在本文探索的时间范围内具有相对化学稳定性。唯一的显著变化发生在分子量和总表面形态。对于大多数植入的血囊，观察到重均分子量适度增加，表明体内存在某种类型的链延伸或支化反应。尽管植入血囊的表面形态通常与其对照血囊相似，但我们有时在使用期间经历最大弯曲的血囊区域的非血液接触表面上观察到有限的点蚀。
In the present study, we investigate the in vivo and in vitro stability of modified poly(urethaneurea) (BioSpan MS/0.4) blood sacs. Blood sacs were utilized primarily in left ventricular assist devices that were implanted in calves for times ranging from 5 to 160 days. Cyclic testing in vitro was also conducted on similar sacs. Various analytical methods were employed to characterize the sacs after in vivo or in vitro service and corresponding retained "control" sacs. These methods included ATR-FTIR spectroscopy, scanning electron microscopy and gel permeation chromatography. In general, the characteristics of implanted and in vitro cycled sacs were similar to their control sacs. Thermal and microtensile properties were unchanged after testing. The same was true for the ATR-FTIR spectra, indicating relative chemical stability for the time frames explored here. The only significant changes occurred in molecular weight and gross surface morphology. A modest increase in weight average molecular weight was observed for most implanted blood sacs, indicating some type of chain extension or branching reaction in vivo. Although the surface morphologies of implanted blood sacs were often similar to their control sacs, we sometimes observed limited pitting on the nonblood contacting surfaces in regions of the sac that experience maximum bending during service.