One-pot microbial synthesis of 2′-deoxyribonucleoside from glucose, acetaldehyde, and a nucleobase
One-pot microbial synthesis of 2′-deoxyribonucleoside from glucose, acetaldehyde, and a nucleobase
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DOI：
10.1007/s10529-006-9019-5
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发表时间：
2006-06-01
影响因子：
2.7
通讯作者：
Shimizu, Sakayu
中科院分区：
文献类型：
--
作者：
Horinouchi, Nobuyuki;Ogawa, Jun;Shimizu, Sakayu
以葡萄糖、乙醛和碱基为原料，建立了一锅法合成2‘-脱氧核糖核苷的方法。酿酒酵母(Saccharmyces Cerevisiae)的糖酵解产生三磷酸腺苷(ATP)，用于以葡萄糖为原料通过1，6-二磷酸果糖生产D-甘油醛3-磷酸。生产的D-甘油醛3-磷酸在乙醛和碱基存在下，经2-脱氧核糖-5-磷酸和2-脱氧核糖-1-磷酸转化为2‘-脱氧核糖核苷。以600 mM葡萄糖、333 mM乙醛和100 mM腺嘌呤为原料，在24 h内可合成约33mm2‘-脱氧肌苷，并利用转化子的腺苷脱氨酶活性合成2’-脱氧肌苷。
A one-pot enzymatic synthesis of 2'-deoxyribonucleoside from glucose, acetaldehyde, and a nucleobase was established. Glycolysis by baker's yeast ( Saccharomyces cerevisiae) generated ATP which was used to produce D-glyceraldehyde 3-phosphate production from glucose via fructose 1,6-diphosphate. The D-glyceraldehyde 3-phosphate produced was transformed to 2'-deoxyribonucleoside via 2-deoxyribose 5-phosphate and then 2-deoxyribose 1-phosphate in the presence of acetaldehyde and a nucleobase by deoxyriboaldolase, phosphopentomutase expressed in Escherichia coli, and a commercial nucleoside phosphorylase. About 33 mM 2'-deoxyinosine was produced from 600 mM glucose, 333 mM acetaldehyde and 100 mM adenine in 24 h. 2'-Deoxyinosine was produced from adenine due to the adenosine deaminase activity of E. coli transformants.