Depletion of bitter taste transduction leads to massive spermatid loss in transgenic mice
Depletion of bitter taste transduction leads to massive spermatid loss in transgenic mice
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DOI：
10.1093/molehr/gas005
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发表时间：
2012-06-01
影响因子：
4
通讯作者：
Zhou, Minliang
中科院分区：
文献类型：
--
作者：
Li, Feng;Zhou, Minliang
苦味感知是针对摄入有毒有害物质的重要感官输入警告。苦味受体是一个由大约 30 个高度分化的 G 蛋白偶联受体组成的家族，专门在含有 G 蛋白 - 味素亚基的味觉受体细胞中表达，在体外与 - 味素结合，并在功能表达测定中对苦味做出反应。我们构建了味觉受体 2 型成员 5 (T2R5)-Cre/绿色荧光蛋白报告基因转基因小鼠来研究 T2R5 的组织分布。我们的结果表明，这些小鼠中的 Cre 基因表达与味觉组织中 T2R5 的表达一致。更令人惊讶的是，免疫染色和 X-gal 染色显示睾丸中存在 T2R5 表达。 T2R5细胞的消融导致睾丸变小，并从大部分生精小管中去除了精细胞期。在精子发生过程中检测到整个味觉转导级联（味导素、Ggamma13、磷脂酶 C2），而仅在精子细胞后期观察到瞬时受体电位、阳离子通道亚家族 M 成员 5 (Trpm5)。简而言之，我们的结果表明味觉转导级联可能参与精子发生。
Bitter taste perception is an important sensory input warning against the ingestion of toxic and noxious substances. Bitter receptors, a family of approximate to 30 highly divergent G-protein-coupled receptors, are exclusively expressed in taste receptor cells that contain the G-protein -subunit gustducin, bind to -gustducin in vitro, and respond to bitter tastes in functional expression assays. We generated a taste receptor type 2 member 5 (T2R5)-Cre/green fluorescent protein reporter transgenic mouse to investigate the tissue distribution of T2R5. Our results showed that Cre gene expression in these mice was faithful to the expression of T2R5 in taste tissue. More surprisingly, immunostaining and X-gal staining revealed T2R5 expression in the testis. Ablation of T2R5 cells led to a smaller testis and removed the spermatid phase from most of the seminiferous tubules. The entire taste transduction cascade (-gustducin, Ggamma13, phospholipase C2) was detected in spermatogenesis, whereas transient receptor potential, cation channel subfamily M member 5 (Trpm5), was observed only in the later spermatid phase. In short, our results indicate that the taste transduction cascade may be involved in spermatogenesis.