Chasing relationships between nutrition and reproduction: A comparative transcriptome analysis of hepatopancreas and testis from Eriocheir sinensis
Chasing relationships between nutrition and reproduction: A comparative transcriptome analysis of hepatopancreas and testis from Eriocheir sinensis
复制标题
追寻营养与繁殖之间的关系：中华绒螯蟹肝胰腺和睾丸的比较转录组分析
DOI：
10.1016/j.cbd.2009.05.001
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发表时间：
2009-09-01
影响因子：
3
通讯作者：
Wang, Qun
中科院分区：
文献类型：
--
作者：
Jiang, Hui;Yin, Yuxin;Wang, Qun
营养与中华绒螯蟹（Eriocheir sinensis）的繁殖有着密切的关系。螃蟹在肝胰腺中储存了大量的能量，这是为繁殖过程中的大量能量输出和消耗做好准备的，但内部分子机制一直不清楚。本文首次报道了中华绒螯蟹肝胰腺与精巢的关系。从一级健康雄性中华绒螯蟹中获得了6287个高质量的EST，共代表3829个单基因。本研究对大肠杆菌肝胰腺和精巢的基因本体和主要代谢过程进行了研究。中国产。基因可能在肝胰腺中表达频率较高且定位于肝胰腺，而在精巢中表达的基因可能丰富，具有多种功能。EST资源中含有许多营养调控基因，包括精氨酸激酶、瘦素受体样蛋白、精浆糖蛋白120和多种锌指蛋白。EST数据还揭示了热休克蛋白70、睾丸增强基因转录本（TEGT）、细胞周期蛋白K等在生殖调控中发挥重要作用的基因。在这些基因中，Leptin受体样蛋白和vasa样蛋白的同源性分别为：sinensis和其他物种的基因组信息量更大。sinensis。我们确定了17个基因相关的控制营养机制和11个基因参与调节生殖。这项研究为螃蟹营养和繁殖的遗传和分子机制提供了见解。这些信息将有助于优化绒螯蟹养殖的育种。(C)2009 Elsevier Inc. All rights reserved.
There is a delicate relationship between nutrition and reproduction of mitten crab (Eriocheir sinensis). The crabs store significant amounts of energy in hepatopancreas, which is prepared for significant energy output and expenditure during reproduction, but the internal molecular mechanism has never been known. Here we present the first relationship between hepatopancreas and testis of E sinensis. We acquired 6287 high quality expressed sequence tags (EST), representing 3829 unigenes totally, from healthy male mitten crabs of first grade. We investigated the Gene Ontology and the main metabolism processes of hepatopancreas and testis from E. sinensis. Genes most likely expressed more frequently and localized in hepatopancreas, and abundant genes from testis for multiple functions. Many genes important for the nutrition regulation are in the EST resource, including arginine kinase, leptin receptor-like protein, seminal plasma glycoprotein 120, and many kinds of zinc finger proteins. The EST data also revealed genes such as heat shock protein 70, testis enhanced gene transcript (TEGT), Cyclin K, etc. predicted to play important roles in regulation of reproduction mechanisms. Among these genes, alignment of leptin receptor-like protein and vasa-like protein from E. sinensis and other species showed even more genomic information on E. sinensis. We identified seventeen genes relevant to control of nutrition mechanisms and eleven genes involved in regulation of reproduction. And this study provides insights into the genetic and molecular mechanisms of nutrition and reproduction in the crab. Such information would facilitate the optimization of breeding in the aquaculture of mitten crabs. (C) 2009 Elsevier Inc. All rights reserved.