Enhanced TNFα and oxidative stress in patients with heart failure:: effect of TNFα on platelet O2- production
Enhanced TNFα and oxidative stress in patients with heart failure:: effect of TNFα on platelet O2- production
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DOI：
10.1160/th03-02-0105
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发表时间：
2003-08-01
影响因子：
6.7
通讯作者：
Violi, F
中科院分区：
文献类型：
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作者：
De Biase, L;Pignatelli, P;Violi, F
实验研究表明，TNF α，一种促炎细胞因子，可能通过各种机制，包括活性氧的产生，导致心血管功能恶化。TNF α在心力衰竭患者中是否具有促氧化活性，以及最终导致这种作用的机制是什么，尚未得到证实。（38名男性和4名女性，年龄26 - 74岁），患有心力衰竭，继发于特发性扩张型心肌病（n=21）、冠状动脉疾病（n= 15）和瓣膜疾病（n=6），对照组20例，男18例，女2例，年龄49 ~ 67岁。根据NYHA分级，10例患者为1级，9例为11级，15例为III级，8例为IV级。从每例患者中采集血液样本，以评估基础和胶原诱导的血小板O-2生成和血浆TNF α。体内结果显示，与对照组或NYHA I-II级患者相比，NYHA III-IV级患者的血小板O-2（-）生成和血浆TNF α水平增加（p
Experimental studies have suggested that TNFalpha, a pro-inflammatory cytokine, may contribute to the deterioration of cardiovascular function through various mechanisms, including the generation of reactive oxygen species. It has not yet been demonstrated whether TNFalpha has prooxidant activity in patients with heart failure, and what the mechanism eventually resulting in this effect are.We analyzed 42 patients (38 men and 4 women, aged 26 to 74 years) with heart failure, secondary to idiopathic dilated cardiomyopathy (n=21), coronary artery disease (n= 15), and valve disease (n=6), and 20 controls (18 men and 2 women, aged 49 to 67 years). Ten patients were in class 1, 9 in class 11, 15 in class III and 8 in class IV according to NYHA Classification. Blood samples were obtained from each patient to evaluate basal and collagen-induced platelet O-2 production, and plasma TNFalpha. In vivo results showed increased platelet O-2(-) production and plasma TNFalpha levels in NYHA class III-IV compared with that in controls or in NYHA I-II (p