DCF1 subcellular localization and its function in mitochondria
DCF1 subcellular localization and its function in mitochondria
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DCF1的亚细胞定位及其在线粒体中的功能
DOI：
10.1016/j.biochi.2017.10.013
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发表时间：
2018-01-01
期刊：
影响因子：
3.9
通讯作者：
Wen, Tieqiao
中科院分区：
文献类型：
--
作者：
Chen, Yanlu;Feng, Ruili;Wen, Tieqiao
树突状细胞因子1（Dendritic cell factor 1，DCF1）是一种跨膜蛋白，在调节神经干细胞分化和树突棘形成中起重要作用。除了其细胞质功能外，DCF1在淀粉样前体蛋白的调节过程中在自噬中起作用。然而，DCF 1的亚细胞定位仍然未知。因此，本研究中，用绿色荧光蛋白标记的DCF 1在HelaS3和HEK 293 T细胞中瞬时表达。结果表明，DCF1广泛表达于线粒体、高尔基体、内质网、内体和溶酶体等细胞器中。碘克沙醇阶梯梯度进一步证实了DCF 1定位于线粒体、核内体、溶酶体、内质网和蛋白酶体。最后，线粒体的功能分析表明，DCF 1影响MGST 1的表达和定位。这项研究提出了一个全面的评估亚细胞定位的DCF 1，这提供了重要的信息，复杂的功能介导的DCF 1。(C)2017 Elsevier B.V.和Societe Francaise de Biochimie et Biologie Moleculaire（SFBBM）。All rights reserved.
Dendritic cell factor 1 (DCF1) is a transmembrane protein that plays important roles in regulating neural stem cell differentiation and dendritic spine formation. Apart from its cytoplasmic functions, DCF1 plays a role in autophagy during the regulation of amyloid precursor proteins. However, the subcellular localization of DCF1 remains unknown. Therefore, in this study, DCF1 tagged with green fluorescent protein was transiently expression in HelaS3 and HEK293T cells. The results showed that DCF1 was widely expressed in different organelles, including the mitochondria, Golgi apparatus, endoplasmic reticulum, endosomes and lysosomes. An iodixanol step gradient further confirmed that DCF1 is localized to the mitochondria, endosomes, lysosomes, endoplasmic reticulum, and proteasome. Finally, functional analysis of the mitochondria revealed that DCF1 affected the expression and localization of MGST1. This study presents a comprehensive evaluation of the subcellular localization of DCF1, which provides important information on complex functions mediated by DCF1. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.