Establishing phenotypic features associated with morbidity in human T-cell lymphotropic virus type 1 infection
Establishing phenotypic features associated with morbidity in human T-cell lymphotropic virus type 1 infection
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DOI：
10.1128/cdli.11.6.1105-1110.2004
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发表时间：
2004-11-01
期刊：
影响因子：
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通讯作者：
Martins-Filho, OA
中科院分区：
文献类型：
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作者：
Brito-Melo, GEA;Souza, JG;Martins-Filho, OA
人类嗜T淋巴细胞病毒1型（HTLV-1）是HTLV-1相关脊髓病/热带痉挛性下肢轻瘫（HT）的病原体。虽然人们普遍认为病毒感染和宿主免疫应答参与了HT的致病机制，但免疫系统在HT的发生和/或维持中的作用仍然未知。我们对两个不同亚群的HTLV-1感染个体的外周血白细胞表型进行了分析，以验证是否存在类似的免疫学改变，可能的HT实验室标志物。在分类为无症状个体、少症状个体和HT个体的HTLV-1感染个体的外周血中评价白细胞群体平衡、T淋巴细胞的活化状态以及T淋巴细胞、单核细胞和中性粒细胞的细胞迁移潜力。数据分析表明，B细胞百分比降低，导致T细胞/B细胞比率增加和CD 8（+）HLA-DR+ T淋巴细胞增加，仅在HT组中可以在两个亚组中鉴定到，这表明其可能用作实验室中使用的HT的潜在免疫学标志物。此外，似然比分析表明，如果HTLV-1感染个体的B细胞百分比低于7.0%，T细胞/B细胞比率高于11，或CD 8（+）HLA-DR+ T淋巴细胞百分比高于70.0%，则该个体属于HT组的机会分别为12倍、13倍或22倍。基于这些数据，我们提出，T细胞/B细胞比例和循环B细胞和活化的CD 8（+）T淋巴细胞在HTLV-1感染患者中的百分比是重要的。免疫学指标可以帮助临床医生监测HTLV-1感染，并区分HT组与无症状和轻微症状组。
The human T-cell lymphotropic virus type 1 (HTLV-1) is the causative agent of HTLV-1-associated myelopathy/tropical spastic paraparesis (HT). Although it is widely believed that virus infection and host immune response are involved in the pathogenic mechanisms, the role of the immune system in the development and/or maintenance of HT remains unknown. We performed an analysis of the peripheral blood leukocyte phenotype for two different subcohorts of HTLV-1-infected individuals to verify the existence of similar immunological alterations, possible laboratory markers for HT. The leukocyte population balance, the activation status of the T lymphocytes, and the cellular migratory potential of T lymphocytes, monocytes, and neutrophils were evaluated in the peripheral blood of HTLV-1-infected individuals classified as asymptomatic individuals, oligosymptomatic individuals, and individuals with HT. Data analysis demonstrated that a decreased percentage of B cells, resulting in an increased T cell/B cell ratio and an increase in the CD8(+) HLA-DR+ T lymphocytes, exclusively in the HT group could be identified in both subcohorts, suggesting its possible use as a potential immunological marker for HT for use in the laboratory. Moreover, analysis of likelihood ratios showed that if an HTLV-1-infected individual demonstrated B-cell percentages lower than 7.0%, a T cell/B cell ratio higher than 11, or a percentage of CD8(+) HLA-DR+ T lymphocytes higher than 70.0%, this individual would have, respectively, a 12-, 13-, or 22-times-greater chance of belonging to the HT group. Based on these data, we propose that the T cell/B cell ratios and percentages of circulating B cells and activated CD8(+) T lymphocytes in HTLV-1-infected patients are important. immunological indicators which could help clinicians monitor HTLV-1 infection and differentiate the HT group from the asymptomatic and oligosymptomatic groups.