Poloxamer 188 enhances endothelial cell survival in bovine corneas in cold storage.
Poloxamer 188 enhances endothelial cell survival in bovine corneas in cold storage.
复制标题
泊洛沙姆 188 可增强冷藏牛角膜内皮细胞的存活率。
DOI：
10.1097/01.ico.0000224638.51224.c1
复制
发表时间：
2006
期刊：
影响因子：
2.8
通讯作者：
Alderton,JanetMarilyn
中科院分区：
文献类型：
--
作者：
Steinhardt,RichardAnthony;Alderton,JanetMarilyn
目的：通过添加一种促进细胞膜修复的化合物，提高角膜内皮细胞在冷保存过程中的存活率。方法：利用荧光分子钙黄蛋白在细胞内的积累来评估细胞存活，钙黄蛋白是Optisol-GS中保存在4℃的牛角膜内皮细胞活力的标志。人工降低细胞膜表面张力对内皮细胞存活的影响也通过添加表面活性剂Poloxamer 188 （PLURONIC F68）进行了分析，该表面活性剂已被证明即使在代谢被抑制的条件下也能重新密封受损的细胞膜。结果：加入1 mg/mL poloxam188后，Optisol-GS中4℃保存的角膜内皮细胞存活率增加。在含有1 mg/mL poloxam188的Optisol-GS中，保存6、9和12天的角膜内皮细胞死亡或缺失的水平低于1%。相比之下，在不含poloxam188的Optisol-GS中，保存9天后死亡或缺失的角膜内皮细胞百分比增加到约4%，在保存4 c后12天死亡或缺失的角膜内皮细胞百分比增加到约10%。结论：表面活性剂poloxam188提高了冷冻保存的牛角膜内皮细胞存活率。本研究的目的是通过维持角膜内皮细胞的活力来延长角膜冷冻保存的有效时间。我们比较了在Optisol-GS中添加或不添加poloxam188（一种促进质膜再密封的添加剂）的牛角膜内皮细胞随时间的活力。如果波洛沙姆188可以在低体温的代谢限制期间促进质膜的重新封闭，那么波洛沙姆188对细胞存活的增强可能在其他情况下有用，例如手术期间的缺血，其中代谢也受到抑制。
Purpose:To improve corneal endothelial cell survival during cold preservation by the addition of a compound that enhances cell membrane repair.Methods:Cell survival was assessed using intracellular accumulation of the fluorescent molecule calcein as a marker for endothelial cell viability in bovine corneas stored at 4 C in Optisol-GS. The effect on endothelial cell survival of artificially lowering the surface tension of the cell membrane was also assayed by the addition of a surfactant, Poloxamer 188 (PLURONIC F68), which has been shown to reseal damaged cell membranes, even under conditions where metabolism is inhibited.Results:Endothelial cell survival in corneas stored at 4 C in Optisol-GS was increased by the addition of 1 mg/mL Poloxamer 188. The level of dead or missing corneal endothelial cells was less than 1% for corneas stored for 6, 9, or 12 days in Optisol-GS with 1 mg/mL Poloxamer 188 at 4 C. In contrast, dead or missing corneal endothelial cell percentages were increased to approximately 4% after 9 days and approximately 10% after 12 days in storage in Optisol-GS without Poloxamer 188 at 4 C.Conclusion:The surfactant Poloxamer 188 enhances endothelial cell survival in cold-stored bovine corneas.The aim of this study was to extend the useful preservation time of cold-stored corneas by maintaining the viability of the endothelium. The viability of the corneal endothelium over time was compared for bovine corneas stored in Optisol-GS with or without Poloxamer 188, an additive that promotes plasma membrane resealing. If Poloxamer 188 can promote plasma membrane resealing during the metabolic restrictions of hypothermia, enhancement of cell survival by Poloxamer 188 might be useful in other circumstances, such as ischemia during surgery, where metabolism is also inhibited.