Adsorption Behavior of Propranolol on Negatively-Charged Liposomes and Its Influence on Membrane Fluidity and Polarity
Adsorption Behavior of Propranolol on Negatively-Charged Liposomes and Its Influence on Membrane Fluidity and Polarity
复制标题
普萘洛尔在负电荷脂质体上的吸附行为及其对膜流动性和极性的影响
DOI：
10.1166/jnn.2017.12823
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hiroshi Umakoshi
中科院分区：
文献类型：
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作者：
Takaaki Ishigami;Chern Michael Sun;Keishi Suga;Yukihiro Okamoto;Hiroshi Umakoshi
研究了R-普萘洛尔（PPL）对带负电荷的脂质体的吸附行为，重点研究了脂质体膜的性质。在带负电荷的DMPA和DMPS脂质体中，膜表面的PPL吸附量较高，而中性DMPC脂质体上的PPL吸附量很少。使用荧光探针，研究了膜流动性和膜极性等膜特性。带负电荷的脂质体上的 PPL 吸附导致膜流动性增加，膜表面发生水合。拉曼光谱分析表明，在 PPL 分子存在下，DMPA 和 DMPS 脂质体的堆积密度降低。认为PPL分子不仅可以通过静电相互作用，还可以通过疏水相互作用、氢键相互作用等其他弱相互作用吸附在膜上。这些发现还表明膜特性（即表面电荷、膜流动性和膜极性）对于理解自组装膜上化学品的吸附行为的重要性。
The adsorption behaviors of R-propranolol (PPL) for the negatively-charged liposomes were studied focusing on the liposome membrane properties. In the case of the negatively-charged DMPA and DMPS liposomes, the adsorbed amount of PPL on the membrane surface was high, while little amount of PPL did on the neutral DMPC liposome. Using fluorescent probes, the membrane properties such as membrane fluidity and membrane polarity were investigated. The PPL adsorption on the negatively-charged liposomes induced an increase in membrane fluidity, and the membrane surface turned to be hydrated. Raman spectroscopic analysis revealed the decrease of packing density of DMPA and DMPS liposomes in the presence of PPL molecules. It is considered that the PPL molecules can adsorb on the membranes through not only electrostatic interaction but also other weak interactions such as hydrophobic interaction and hydrogen bond interaction. These findings also suggest the significance of the membrane properties, i.e., surface charge, membrane fluidity, and membrane polarity, in understanding the adsorption behaviors of chemicals on the self-assembled membranes.