Two untargeted metabolomics reveals yogurt-associated metabolic alterations in women with multiple metabolic disorders from a randomized controlled study
Two untargeted metabolomics reveals yogurt-associated metabolic alterations in women with multiple metabolic disorders from a randomized controlled study
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一项随机对照研究显示，两项非靶向代谢组学揭示了患有多种代谢紊乱的女性与酸奶相关的代谢改变
DOI：
10.1016/j.jprot.2021.104394
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发表时间：
2022
影响因子：
3.3
通讯作者：
Rennan Feng
中科院分区：
文献类型：
--
作者：
Shanshan Du;Yang Chen;Xiaoxue Liu;Zhihong Zhang;Yongshuai Jiang;Yang Zhou;Hongxia Zhang;Qiyang Li;XuemeiWang;Yan Wang;Rennan Feng
酸奶对新陈代谢的有益作用已被广泛报道。然而，很少有研究打算描述酸奶带来的综合代谢变化。在一项随机对照试验中，48名和44名患有代谢综合征和非酒精性脂肪性肝病的肥胖女性分别服用酸奶和牛奶(220g/d)，为期24周(在http://www.chictr.org.cn注册为ChiCTR-IPR-15006801)。干预前后采集空腹血清样本，进行基于~1H核磁共振(核磁共振)和超高效液相色谱-电喷雾电离飞行时间质谱仪(UPLC-Q-TOF-MS)的全球非靶向代谢组学研究(正负离子模式)。进行多变量统计分析和通径分析。在1H核磁共振和UPLC-Q-TOF-MS代谢组学中，在基线时两组之间没有观察到聚集。而干预后表现出明显的聚集性，酸奶组与牛奶组的代谢状态有显著差异。确定了对分类分离贡献最大的代谢物，并将其纳入通径分析。酸奶干预后，氨基酸代谢、脂肪酸氧化、胆固醇分解代谢和胆碱代谢途径发生了显著变化。这项研究通过两种代谢组学方法揭示了酸奶响应的综合代谢变化，暗示了酸奶对抗代谢紊乱的潜在机制。试验注册中国临床试验注册，CHICCTR-IPR-15006801。2015年7月20日注册的http://www.chictr.org.cn/ChiCTR-IPR-15006801意义来自前瞻性研究和我们的随机临床试验的综述表明，酸奶对多种代谢紊乱具有保护作用。然而，他们关注的目标是葡萄糖、脂肪和其他代谢指标，这些指标只是人类新陈代谢的一部分，未能显示出酸奶的综合代谢特征。因此，我们在目前的随机临床试验中应用了两种全球性的、非靶向的代谢组学，试图揭示酸奶对患有多种代谢紊乱的肥胖女性的显著代谢变化，并探讨酸奶的潜在生物学机制。这一发现将更全面地揭示酸奶如何影响宿主新陈代谢，并为饮食预防慢性病提供理论基础。
The beneficial role of yogurt on metabolic profile has been widely reported. Yet, few studies have intended to describe the integrated metabolic alterations in response to yogurt. Yogurt and milk (220 g/d) were given to 48 and 44 obese women with metabolic syndrome and nonalcoholic fatty liver disease for 24 weeks in a randomized controlled trial (registered at http://www.chictr.org.cn as ChiCTR-IPR-15006801). Fasting serum samples were collected before and after intervention for global, untargeted metabolomics based on1H nuclear magnetic resonance (NMR) and ultra-high-performance liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry (UPLC-Q-TOF-MS) (in positive and negative ion modes). Multivariable statistical analysis and pathway analysis were conducted. In both1H NMR and UPLC-Q-TOF-MS metabolomics, no clustering was observed between the two groups at baseline. While, a clear clustering was shown after intervention, and the yogurt group had significantly different metabolic status from the milk. The metabolites that contributed mostly to class separation were identified, and involved into pathway analysis. Pathways on amino acids metabolism, fatty acid oxidation, cholesterol catabolism and choline metabolism significantly changed after yogurt intervention. The study revealed the integrated metabolic alterations in response to yogurt via two metabolomics approaches, suggesting the potential mechanisms of yogurt against metabolic disorders.Trial registrationChinese Clinical Trial Registry, ChiCTR-IPR-15006801. Registered 20 July 2015, http://www.chictr.org.cn/ ChiCTR-IPR-15006801.SignificanceBoth review from prospective studies and our randomized clinical trial showed the protective role of yogurt against multiple metabolic disorders. However, they were focus on targeted glucose, lipid, and other metabolic indicators, which were only part of human metabolism, failing to show an integrated metabolic feature on yogurt. Therefore, two global, untargeted metabolomics were applied in our current randomized clinical trial, trying to uncover the significant metabolic alterations characterizing the effects of yogurt on obese women with multiple metabolic disorders, and to explore the potential biological mechanisms of yogurt. The finding will shed light on a more comprehensive picture of how yogurt affects host metabolism, and provide theoretical foundation for dietary prevention of chronic diseases.