Role of extracellular Ca2+ and calcium channel activated by a G protein-coupled receptor regulating pheromone production in Helicoverpa zea (Lepidoptera: Noctuidae)
Role of extracellular Ca2+ and calcium channel activated by a G protein-coupled receptor regulating pheromone production in Helicoverpa zea (Lepidoptera: Noctuidae)
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DOI：
10.1603/0013-8746(2006)99
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发表时间：
2006-09-01
影响因子：
2.3
通讯作者：
Jurenka, Russell A.
中科院分区：
文献类型：
--
作者：
Choi, Man-Yeon;Jurenka, Russell A.
研究了信息素生物合成激活神经肽(PBAN)对玉米蛾(Helicoverpa zea(Boddie))(鳞翅目：夜蛾科)分离信息素腺体的生理作用，以及钙通道在刺激信息素生物合成中的作用。剂量-反应曲线表明，PBAN的刺激水平在10(-11)M时达到最低水平，PBAN的活动需要细胞外钙离子的内流。从PBAN激活受体到胞外钙离子内流的时间延迟长达7.5min。这些结果表明，PBAN受体的激活在一段时间内维持钙通道的激活。在表达克隆的PBAN受体的Sf9细胞中也发现了类似的结果。这表明Sf9细胞有一个与受体偶联的钙通道，其方式与信息素腺体细胞相似。为确定钙通道的类型，用PBAN和有机钙通道阻断剂孵育离体腺。L型电压门控性钙通道阻滞剂和非选择性钙通道阻滞剂均不抑制信息素的产生。然而，钾通道阻滞剂奎尼丁抑制信息素的产生。这些结果表明，信息素腺体内受体激活的钙通道可能不是突触激活的钙通道，而是一种非选择性离子通道。我们的结果进一步揭示了PBAN在飞蛾信息素生物合成的信号转导中与钙通道的作用。
The physiological action of pheromone biosynthesis activating neuropeptide (PBAN) on isolated pheromone glands of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) was investigated with regard to the role of the Ca2+ channel in stimulating pheromone biosynthesis. A dose-response profile indicated that the minimum level of PBAN stimulation was achieved at 10(-11) M. PBAN activity requires the influx of extracellular Ca2+. A time delay of up to 7.5 min from receptor activation by PBAN and influx of extracellular Ca2+ was determined. These results indicate that activation of the PBAN-receptor maintains the activation of a Ca2+ channel for a period of time. Similar results were found using transfected Sf9 cells expressing the cloned PBAN-receptor. This indicates that the Sf9 cells have a Ca2+ channel that is coupled to the receptor in a similar way as in the pheromone gland cells. To determine the type of Ca2+ channel, isolated glands were incubated with PBAN and organic Ca2+ channel blockers. L-type voltage-gated Ca2+ or nonselective Ca2+ channel blockers did not inhibit pheromone production. However, quinidine, a potassium channel blocker, inhibited pheromone production. These results indicate that the receptor-activated Ca2+ channel in the pheromone gland may not be avoltage-activated Ca2+ channel but rather a nonselective ion channel. Our results provide further insight into the action of PBAN with regard to the Ca2+ channel in the signal transduction of pheromone biosynthesis in moths.