Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd3+ spin labels
Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd3+ spin labels
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DOI：
10.1039/c2cp40219j
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发表时间：
2012-01-01
影响因子：
3.3
通讯作者：
Goldfarb, Daniella
中科院分区：
文献类型：
--
作者：
Kaminker, Ilia;Yagi, Hiromasa;Goldfarb, Daniella
脉冲DER(双电子-电子共振)技术常用于测量生物大分子中特定位置之间的纳米距离。在这项工作中，我们将这种方法的适用性扩展到具有混合自旋标记的蛋白质组件中的高场距离测量，即氮氧自旋标记和Gd3+标记。我们证明了光谱选择两个氮氧化物自旋标记物、一个氮氧化物自旋标记物和一个Gd3+离子以及两个Gd3+离子之间距离分布的可能性。GD3+-氮氧化物鹿的测量结合了高灵敏度和数据分析的简便性，在仪器上有所改进，因此在W波段远程测量(6 Nm)方面具有很高的潜力。
The pulse DEER (Double Electron-Electron Resonance) technique is frequently applied for measuring nanometer distances between specific sites in biological macromolecules. In this work we extend the applicability of this method to high field distance measurements in a protein assembly with mixed spin labels, i.e. a nitroxide spin label and a Gd3+ tag. We demonstrate the possibility of spectroscopic selection of distance distributions between two nitroxide spin labels, a nitroxide spin label and a Gd3+ ion, and two Gd3+ ions. Gd3+-nitroxide DEER measurements possess high potential for W-band long range distance measurements (6 nm) by combining high sensitivity with ease of data analysis, subject to some instrumental improvements.