Runx1 and Runx3 Are Downstream Effectors of Nanog in Promoting Osteogenic Differentiation of the Mouse Mesenchymal Cell Line C3H10T1/2
Runx1 and Runx3 Are Downstream Effectors of Nanog in Promoting Osteogenic Differentiation of the Mouse Mesenchymal Cell Line C3H10T1/2
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DOI：
10.1089/cell.2014.0059
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发表时间：
2015-06-01
影响因子：
1.6
通讯作者：
Ogasawara, Toru
中科院分区：
文献类型：
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作者：
Saito, Tadahito;Ohba, Shinsuke;Ogasawara, Toru
以前，我们报道了转录因子Nanog，它维持胚胎干细胞（ESCs）的自我更新，促进小鼠间充质细胞系C3 H10 T1/2的成骨分化，通过基因组重编程过程。为了阐明骨髓间充质干细胞（mesenchymal stem cells，MSCs）多能性的机制，为骨再生医学提供新的途径，我们尝试鉴定Nanog促进小鼠骨髓间充质干细胞成骨分化的下游效应因子。我们证明Runx 1和Runx 3是Nanog的下游效应子，特别是在小鼠间充质细胞系C3 H10 T1/2的早期和中期成骨分化中。
Previously, we reported that the transcription factor Nanog, which maintains the self-renewal of embryonic stem cells (ESCs), promotes the osteogenic differentiation of the mouse mesenchymal cell line C3H10T1/2 through a genome reprogramming process. In the present study, to clarify the mechanism underlying the multipotency of mesenchymal stem cells (MSCs) and to develop a novel approach to bone regenerative medicine, we attempted to identify the downstream effectors of Nanog in promoting osteogenic differentiation of mouse mesenchymal cells. We demonstrated that Runx1 and Runx3 are the downstream effectors of Nanog, especially in the early and intermediate osteogenic differentiation of the mouse mesenchymal cell line C3H10T1/2.