Decreased Dicer expression is linked to increased expression of co-stimulatory molecule CD80 on B cells in multiple sclerosis.
Decreased Dicer expression is linked to increased expression of co-stimulatory molecule CD80 on B cells in multiple sclerosis.
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DOI：
10.1177/1352458514560923
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Balashov KE
中科院分区：
文献类型：
--
作者：
Aung LL;Balashov KE
MS 是一种免疫介导的中枢神经系统炎症性疾病。根据 B 细胞消融的临床试验，B 细胞与疾病发病机制密切相关。越来越多的证据表明 microRNA 与免疫系统的调节有关。 Dicer 是一种参与 microRNA 生物发生的关键酶，对于正常 B 细胞功能是必需的。确定 B 细胞中 Dicer 表达是否受损，以及是否与 MS 患者中共刺激分子表达增加有关。从多发性硬化症患者和健康受试者中分离出 B 细胞。测试了 Dicer 和共刺激分子 CD80 和 CD86 的表达。研究了 Dicer 调节对 B 细胞中 CD80 和 CD86 表达的影响。与健康受试者相比，多发性硬化症患者的 B 细胞中 Dicer 表达降低，但单核细胞中没有降低。与健康受试者相比，MS 患者 B 细胞上的 CD80 和 CD86 表达增加。小干扰RNA抑制B细胞中的Dicer表达导致CD80表达增加。 Dicer 表达降低，在机制上与 MS 患者 B 细胞中共刺激分子 CD80 表达增加有关。这可能有助于激活多发性硬化症的免疫反应。
MS is an immune-mediated inflammatory disease of the CNS. B cells have been strongly implicated in disease pathogenesis based on clinical trials with B cell ablation. There is a growing body of evidence linking microRNAs with regulation of the immune system. Dicer, a key enzyme involved in microRNA biogenesis, is necessary for normal B cell function. To determine whether Dicer expression is impaired in B cells and is linked to increased expression co-stimulatory molecules in MS patients. B cells were separated from MS patients and healthy subjects. Expression of Dicer and co-stimulatory molecules CD80 and CD86 was tested. The effect of Dicer modulation on CD80 and CD86 expression in B cells was studied. Dicer expression was decreased in B cells but not in monocytes of MS patients compared to healthy subjects. CD80 and CD86 expression was increased on B cells of MS patients compared to healthy subjects. Inhibition of Dicer expression in B cells by small interfering RNA led to increased expression of CD80. Dicer expression is decreased and is mechanistically linked to increased expression of co-stimulatory molecule CD80 in B cells of MS patients. This may contribute to activation of immune responses in MS.
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影响因子：
15.9
作者：
Genc, K;Dona, DL;Reder, AT
通讯作者：
Reder, AT
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作者：
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64.5
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32.4
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11.2
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Polman CH;Reingold SC;Banwell B;Clanet M;Cohen JA;Filippi M;Fujihara K;Havrdova E;Hutchinson M;Kappos L;Lublin FD;Montalban X;O'Connor P;Sandberg-Wollheim M;Thompson AJ;Waubant E;Weinshenker B;Wolinsky JS
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Wolinsky JS