Concurrent occurrence of 3 beta,12 alpha-dihydroxy-5-cholenoic acid associated with 3 beta-hydroxy-5-cholenoic acid and their preferential urinary excretion in liver diseases.
Concurrent occurrence of 3 beta,12 alpha-dihydroxy-5-cholenoic acid associated with 3 beta-hydroxy-5-cholenoic acid and their preferential urinary excretion in liver diseases.
复制标题
肝脏疾病中 3β,12α-二羟基-5-胆烯酸与 3β-羟基-5-胆烯酸的同时出现及其优先尿排泄。
DOI：
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发表时间：
1989
影响因子：
6.5
通讯作者：
H. Miyazaki
中科院分区：
文献类型：
--
作者：
J. Shoda;T. Osuga;K. Matsuura;R. Mahara;M. Tohma;N. Tanaka;Y. Matsuzaki;H. Miyazaki
采用气-液-质联用(GLC-MS)技术对肝病患者血清中的3β-羟基-(Delta 5-3β-ol)、3β，12α-二羟基(Delta 5-3β，12α-ol)、3β，7α-二羟基(Delta 5-3β，7α-ol)和3β，7-β-二羟基-(Delta 5-3β，7β-ol)5-胆烯酸进行了鉴定。在这些罕见的3β-羟基-5-烯代谢物中，在肝病(胆汁淤积症、肝硬变、慢性肝炎、急性肝炎)患者的尿液中发现了主要的5-3β-醇和5-3β，12α-醇。另有3种β-二羟基-5-烯代谢物，即Delta 5-3β，7α-ol和Delta 5-3β，7β-ol是尿液中的次要成分。尿Delta5-3β-ol、Delta 5-3β、12α-ol与血清、尿总胆汁酸、肝功能呈正相关，提示其升高程度与肝病严重程度密切相关。与胆汁、门静脉和肝静脉血清及肝组织相比，尿液中以硫酸盐结合物的形式存在最丰富的5-3β-醇和5-3β，12α-醇。这3种β-羟基-5-烯不饱和胆汁酸对胆汁排泄和肝脏提取的损害比胆酸和鹅去氧胆酸更严重，效率更低。
3 beta-Hydroxy-(delta 5-3 beta-ol), 3 beta,12 alpha-dihydroxy-(delta 5-3 beta,12 alpha-ol), 3 beta,7 alpha-dihydroxy-(delta 5-3 beta,7 alpha-ol) and 3 beta,7 beta-dihydroxy-(delta 5-3 beta,7 beta-ol) 5-cholenoic acids were identified in patients with liver diseases by gas-liquid chromatography-mass spectrometry (GLC-MS). Of these unusual 3 beta-hydroxy-5-en-metabolites, delta 5-3 beta-ol and delta 5-3 beta,12 alpha-ol were found as major components in the urine of patients with liver diseases (cholestasis, liver cirrhosis, chronic hepatitis, acute hepatitis). Other 3 beta-dihydroxy-5-en-metabolites, delta 5-3 beta,7 alpha-ol and delta 5-3 beta,7 beta-ol, were found as minor components in the urine. The levels of delta 5-3 beta-ol and delta 5-3 beta,12 alpha-ol in urine were correlated with their levels in serum, with total bile acids in the urine, and with liver function, implying that the degree of their increment correlated well with the severity of liver diseases. The most abundant amounts of delta 5-3 beta-ol and delta 5-3 beta,12 alpha-ol were found in the urine as sulfate conjugates in comparison with bile, portal and hepatic venous sera, and liver tissue of the patients. The biliary excretion and hepatic extraction of these 3 beta-hydroxy-5-en-unsaturated bile acids were more impaired and inefficient than those of cholic and chenodeoxycholic acids.
DOI：
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发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kok,E;Burstein,S;Javitt,NB;Gut,M;Byon,CY
通讯作者：
Byon,CY