In vitro response of tumour cells to non-uniform irradiation
In vitro response of tumour cells to non-uniform irradiation
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DOI：
10.1088/0031-9155/50/13/005
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发表时间：
2005-07-07
影响因子：
3.5
通讯作者：
Jackson, M
中科院分区：
文献类型：
--
作者：
Suchowerska, N;Ebert, MA;Jackson, M
本研究利用克隆源性细胞存活情况，检验了均匀照射和非均匀照射下肿瘤细胞反应的差异。细胞用6 MV X射线调强束进行照射，放在一个大烧瓶中（即细胞间通讯是可能的），或者放在三个小烧瓶中（即细胞间通讯在剂量梯度上受到抑制）。对于非小细胞肺癌和黑色素瘤细胞系，在整个细胞培养过程中，自由通讯的细胞培养物与在剂量不均匀情况下通讯受抑制的细胞培养物之间的剂量反应存在显著差异。通讯细胞在照射区域的低剂量区存活率较低，但在高剂量区存活率提高。一般来说，在整个细胞培养过程中，对非均匀照射的反应似乎“平均化”了。当细胞间通讯被抑制时，这种情况没有出现。这些结果为有关旁观者效应和细胞反应相互依赖性的大量证据提供了支持。
This study examines differences in tumour cellular response using clonogenic cell survival between uniform and non-uniform irradiation. Cells were irradiated with a 6 MV x-ray intensity-modulated beam, in a. single large flask (i.e. intercellular communication is possible) or in three small flasks (i.e. intercellular communication is inhibited across the dose gradient). For non-small-cell lung cancer and melanoma cell lines, the dose response over the entire cell culture was significantly different between freely communicating cell cultures and those with inhibited communication across the dose non-uniformity. Communicating cells exhibited poorer survival in the low dose region of the field but improved survival in the high dose region. In general, the response to non-uniform irradiation appeared to 'average out' over the entire cell culture. This was not seen when intercellular communication was inhibited. The results add strength to the body of evidence regarding bystander effects and the inter-dependence of cellular response.