Dissection of the neuron network in the catfish inner retina. V. Interactions between NA and NB amacrine cells.
Dissection of the neuron network in the catfish inner retina. V. Interactions between NA and NB amacrine cells.
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鲶鱼视网膜内神经元网络的解剖。
DOI：
10.1152/jn.1990.63.1.120
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发表时间：
1990
影响因子：
2.5
通讯作者：
Naka,KI
中科院分区：
文献类型：
--
作者：
Sakai,HM;Naka,KI
1.同时细胞内记录从两个相邻的N无长突细胞，一个ON无长突（NA）细胞和其他OFF无长突（NB）细胞。将外源性电流注入一个无长突细胞，并从另一个无长突细胞记录所产生的细胞内反应。测试信号包括：1）单频正弦波，2）去极化或超极化脉冲，或3）白噪声调制电流。在一些细胞对中，在黑暗中以及在稳定的背景照明下测量膜噪声。2.注入NA细胞的电流脉冲引起NB细胞的阻尼振荡。通过将注入NA细胞的白噪声电流与NB细胞的诱发反应互相关得到的一阶核是一个大的去极化，然后是阻尼振荡。每对的振荡频率略有不同，但平均为35 Hz。3.注入NB细胞的电流脉冲诱发NA细胞的非常小幅度的符号反转反应（超极化）。类似地，一阶核是非常小振幅的超极化。4. NA和NB细胞在黑暗中或在稳定照明期间记录的膜噪声的功率谱通常在35 Hz处显示峰值。在黑暗中，这种膜噪声在NA细胞和NB细胞之间协同作用。此外，在黑暗中在NA和NB细胞中观察到的膜波动是异相的。5. NA和NB细胞之间的传输主要是由线性分量占，然而，一个非常小的，但显着的二阶和三阶非线性也产生了。6.这些结果表明，相反的反应极性的无长突细胞之间发生的相互作用要比相同的反应极性的细胞之间的那些复杂得多，并且在视网膜内层的神经回路积极地控制在黑暗中以及在光刺激的存在下ON和OFF通道之间的相互作用。
1. Simultaneous intracellular recordings were made from two neighboring N amacrine cells, one an ON amacrine (NA) cell and the other an OFF amacrine (NB) cell. Extrinsic current was injected into one amacrine cell, and the resulting intracellular responses were recorded from the other amacrine cell. Test signals included 1) a single-frequency sinusoid, 2) a depolarizing or hyperpolarizing pulse, or 3) a white-noise modulated current. In some cell pairs, membrane noise was measured in the dark as well as under a steady background illumination. 2. Current pulses injected into a NA cell evoked a damped oscillation from a NB cell. The first-order kernel derived by cross-correlating the white-noise current injected into a NA cell against the evoked response from a NB cell was a large depolarization followed by a damped oscillation. The frequency of oscillations varied slightly from pair to pair but averaged 35 Hz. 3. Current pulses injected into a NB cell evoked a sign-inverting response (hyperpolarization) of very small amplitude from a NA cell. Similarly, the first-order kernel was a hyperpolarization of very small amplitude. 4. The power spectrum of the membrane noise recorded from NA and NB cells in the dark or during steady illumination often showed a peak at 35 Hz. Such membrane noise synchronizes synergistically among NA cells and among NB cells in the dark. In addition, the membrane fluctuations seen in NA and NB cells in the dark were out of phase. 5. Transmission between NA and NB cells was largely accounted for by a linear component; however, a very small but significant second- and third-order nonlinearity was also generated. 6. These results show that the interactions occurring between amacrine cells of opposite response polarity are much more complex than those between cells of the same response polarity and that the neural circuitry in the inner retina actively controls interactions between ON and OFF channels in the dark as well as in the presence of light stimuli.